Curation Details
Interaction ID: IM-22230-3

Unique identifier for interactor Auniprotkb:P0DPI0
Unique identifier for interactor Buniprotkb:Q9Z2I6
Alternative identifier for interactor Aintact:EBI-8178893
uniprotkb:P18639
intact:MINT-1795451
uniprotkb:P01561
uniprotkb:A5HZZ9
uniprotkb:A7G1U9
uniprotkb:P10845
Alternative identifier for interactor Bintact:EBI-8178859
intact:MINT-1795475
ensembl:ENSRNOP00000024459.3
Aliases for Apsi-mi:bxa1_clobo(display_long)
uniprotkb:botA(gene name)
psi-mi:botA(display_short)
uniprotkb:atx(gene name synonym)
uniprotkb:Bontoxilysin-A(gene name synonym)
uniprotkb:Botulinum neurotoxin type A1(gene name synonym)
uniprotkb:bonT(gene name synonym)
Aliases for Bpsi-mi:sv2c_rat(display_long)
uniprotkb:Sv2c(gene name)
psi-mi:Sv2c(display_short)
Interaction detection methodspsi-mi:"MI:0096"(pull down)
First author- (2014)
Identifier of the publicationimex:IM-22230
pubmed:24583011
NCBI Taxonomy identifier for interactor Ataxid:1491(clobo)
taxid:1491(Clostridium botulinum)
NCBI Taxonomy identifier for interactor Btaxid:10116(rat)
taxid:10116("Rattus norvegicus (Rat)")
Interaction typespsi-mi:"MI:0407"(direct interaction)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-9118895
imex:IM-22230-3
Confidence scoreintact-miscore:0.62
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role APrey
Experimental role BBait
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:f2
comment:"\"This direct protein-protein interaction is clearly demonstrated in a GST pull down assay employing full-length recombinant H6tBoNTA and rat GST-rSV2C 454-579 (Fig. 1A). The weaker binding of HCA to recombinant rat GST-rSV2A 468-594 is also shown (Fig. S1) whereas binding to non-glycosylated rSV2B is hardly detectable. Well characterised neutralising monoclonal antibodies directed against different epitopes within the HC-fragment of BoNT/A [25-29] were screened for interference with the SV2C interaction\""
curation depth:imex curation
full coverage:Only protein-protein interactions
NCBI Taxonomy identifier for the host organismtaxid:-1(in vitro)
taxid:-1(In vitro)
Parameters of the interaction-
Creation date2014/02/06
Update date2024/11/14
negative Boolean valuefalse
Feature(s) for interactor Amutation decreasing interaction:1131-1131
his tag:?-?
Feature(s) for interactor Bbinding-associated region:454-579
glutathione s tranferase tag:?-?
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor APredetermined participant
Participant identification method for interactor BPredetermined participant