Curation Details
Interaction ID: MINT-6175286

Unique identifier for interactor Auniprotkb:Q13616
Unique identifier for interactor Buniprotkb:P61081
Alternative identifier for interactor Aintact:EBI-359390
uniprotkb:O60719
uniprotkb:Q08AL6
uniprotkb:Q8IYW1
uniprotkb:D3DWG3
Alternative identifier for interactor Bintact:EBI-1041660
uniprotkb:O76069
uniprotkb:Q8VC50
Aliases for Apsi-mi:cul1_human(display_long)
uniprotkb:CUL1(gene name)
psi-mi:CUL1(display_short)
Aliases for Bpsi-mi:ubc12_human(display_long)
uniprotkb:UBE2M(gene name)
psi-mi:UBE2M(display_short)
uniprotkb:UBC12(gene name synonym)
uniprotkb:Ubiquitin-conjugating enzyme E2 M(gene name synonym)
uniprotkb:NEDD8 carrier protein(gene name synonym)
Interaction detection methodspsi-mi:"MI:0415"(enzymatic study)
First authorKumar et al. (2007)
Identifier of the publicationpubmed:17914462
imex:IM-14019
mint:MINT-6175264
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0567"(neddylation reaction)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-7629112
mint:MINT-6175286
imex:IM-14019-1
Confidence scoreintact-miscore:0.79
Complex expansion-
Biological role AEnzyme target
Biological role BEnzyme
Experimental role ANeutral component
Experimental role BNeutral component
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:F5A
comment:"\"In vitro neddylation was carried out in the presence of H2O2 using purified Ubc12, a complex containing Roc1 and the C-terminal portion (residues 324 776) of Cul-1, and 32P-NEDD8.\""
comment:homomint
comment:mint
dataset:Virus - Publications including interactions involving viral proteins
full coverage:Only protein-protein interactions
curation depth:imex curation
NCBI Taxonomy identifier for the host organismtaxid:-1(in vitro)
taxid:-1(In vitro)
Parameters of the interaction-
Creation date2008/02/07
Update date2014/10/16
negative Boolean valuefalse
Feature(s) for interactor Abinding-associated region:324-776(MINT-6175297)
Feature(s) for interactor Btag:?-?(MINT-6175289)
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor APredetermined participant
Participant identification method for interactor BPredetermined participant