Curation Details
Interaction ID: IM-28387-1

Unique identifier for interactor Auniprotkb:P14780
Unique identifier for interactor Buniprotkb:Q9ZFS6
Alternative identifier for interactor Aintact:EBI-1382326
Alternative identifier for interactor Bintact:EBI-26361542
Aliases for Apsi-mi:mmp9_human(display_long)
uniprotkb:MMP9(gene name)
uniprotkb:CLG4B(gene name synonym)
uniprotkb:92 kDa type IV collagenase(gene name synonym)
uniprotkb:92 kDa gelatinase(gene name synonym)
uniprotkb:Gelatinase B(gene name synonym)
Aliases for Bpsi-mi:q9zfs6_staau(display_long)
uniprotkb:set3(gene name)
uniprotkb:NCTC7878_00467(orf name)
Interaction detection methodspsi-mi:"MI:0096"(pull down)
First authorKurisaka et al. (2018)
Identifier of the publicationimex:IM-28387
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:1280(staau)
taxid:1280(Staphylococcus aureus)
Interaction typespsi-mi:"MI:0915"(physical association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-26361569
Confidence scoreintact-miscore:0.54
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role APrey
Experimental role BBait
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:f2a f4b f5 f7b
comment:"\"he pull‐down assay using GST‐SSL5‐agarose in combination with zymography or western blotting showed that immobilized SSL5 bound to MMP‐9 derived from CHO‐K1 cells but did not bind to MMP‐9 from Lec2 mutant cells (Fig. 5).\""
comment:"\"Pull‐down assay clearly showed that GST‐SSL5‐agarose failed to coprecipitate neuraminidase‐treated MMP‐9 (Fig. 4b).\""
comment:"\"The pull‐down assay with GST‐SSL5‐agarose showed that MMP‐9 from benzyl‐GalNAc‐treated THP‐1 cells possessed weaker reactivity with GST‐SSL5 (Fig. 7b), suggesting that O‐gycans of MMP‐9 play a crucial role in its binding to GST‐SSL5.\""
full coverage:Only protein-protein interactions
curation depth:imex curation
NCBI Taxonomy identifier for the host organismtaxid:-1(in vitro)
taxid:-1(In vitro)
Parameters of the interaction-
Creation date2020/09/29
Update date2020/09/29
negative Boolean valuefalse
Feature(s) for interactor A-
Feature(s) for interactor Bglutathione s tranferase tag:?-?
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor AWestern blot
Participant identification method for interactor BWestern blot