Curation Details
Interaction ID: IM-28387-1

Unique identifier for interactor Auniprotkb:Q9ZFS6
Unique identifier for interactor Buniprotkb:P14780
Alternative identifier for interactor Aintact:EBI-26361542
Alternative identifier for interactor Bintact:EBI-1382326
Aliases for Apsi-mi:q9zfs6_staau(display_long)
uniprotkb:set3(gene name)
uniprotkb:NCTC7878_00467(orf name)
Aliases for Bpsi-mi:mmp9_human(display_long)
uniprotkb:MMP9(gene name)
uniprotkb:CLG4B(gene name synonym)
uniprotkb:92 kDa type IV collagenase(gene name synonym)
uniprotkb:92 kDa gelatinase(gene name synonym)
uniprotkb:Gelatinase B(gene name synonym)
Interaction detection methodspsi-mi:"MI:0096"(pull down)
First authorKurisaka et al. (2018)
Identifier of the publicationimex:IM-28387
NCBI Taxonomy identifier for interactor Ataxid:1280(staau)
taxid:1280(Staphylococcus aureus)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0915"(physical association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-26361569
Confidence scoreintact-miscore:0.54
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role ABait
Experimental role BPrey
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:f2a f4b f5 f7b
comment:"\"he pull‐down assay using GST‐SSL5‐agarose in combination with zymography or western blotting showed that immobilized SSL5 bound to MMP‐9 derived from CHO‐K1 cells but did not bind to MMP‐9 from Lec2 mutant cells (Fig. 5).\""
comment:"\"Pull‐down assay clearly showed that GST‐SSL5‐agarose failed to coprecipitate neuraminidase‐treated MMP‐9 (Fig. 4b).\""
comment:"\"The pull‐down assay with GST‐SSL5‐agarose showed that MMP‐9 from benzyl‐GalNAc‐treated THP‐1 cells possessed weaker reactivity with GST‐SSL5 (Fig. 7b), suggesting that O‐gycans of MMP‐9 play a crucial role in its binding to GST‐SSL5.\""
full coverage:Only protein-protein interactions
curation depth:imex curation
NCBI Taxonomy identifier for the host organismtaxid:-1(in vitro)
taxid:-1(In vitro)
Parameters of the interaction-
Creation date2020/09/29
Update date2020/09/29
negative Boolean valuefalse
Feature(s) for interactor Aglutathione s tranferase tag:?-?
Feature(s) for interactor B-
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor AWestern blot
Participant identification method for interactor BWestern blot