Curation Details
Interaction ID: IM-23071-1

Unique identifier for interactor Auniprotkb:P27361
Unique identifier for interactor Buniprotkb:Q8N490
Alternative identifier for interactor Aintact:EBI-73995
uniprotkb:B0LPG3
uniprotkb:A8CZ58
uniprotkb:Q8NHX1
ensembl:ENSP00000263025.4
Alternative identifier for interactor Bintact:EBI-746368
uniprotkb:A8K1F2
uniprotkb:Q96A48
uniprotkb:Q9BU26
uniprotkb:Q9NSX4
uniprotkb:Q9ULN6
uniprotkb:Q9Y4T1
ensembl:ENSP00000273077.4
Aliases for Apsi-mi:mk03_human(display_long)
uniprotkb:ERT2(gene name synonym)
uniprotkb:MAP kinase isoform p44(gene name synonym)
uniprotkb:Microtubule-associated protein 2 kinase(gene name synonym)
uniprotkb:Extracellular signal-regulated kinase 1(gene name synonym)
uniprotkb:Insulin-stimulated MAP2 kinase(gene name synonym)
uniprotkb:p44-ERK1(gene name synonym)
uniprotkb:MAPK3(gene name)
psi-mi:MAPK3(display_short)
uniprotkb:ERK1(gene name synonym)
uniprotkb:PRKM3(gene name synonym)
Aliases for Bpsi-mi:pnkd_human(display_long)
uniprotkb:Paroxysmal nonkinesiogenic dyskinesia protein(gene name synonym)
uniprotkb:Myofibrillogenesis regulator 1(gene name synonym)
uniprotkb:Trans-activated by hepatitis C virus core protein 2(gene name synonym)
uniprotkb:PNKD(gene name)
psi-mi:PNKD(display_short)
uniprotkb:KIAA1184(gene name synonym)
uniprotkb:MR1(gene name synonym)
uniprotkb:TAHCCP2(gene name synonym)
uniprotkb:FKSG19(orf name)
uniprotkb:UNQ2491/PRO5778(orf name)
Interaction detection methodspsi-mi:"MI:0006"(anti bait coimmunoprecipitation)
First authorGong et al. (2014)
Identifier of the publicationimex:IM-23071
pubmed:25066297
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0915"(physical association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-9663024
imex:IM-23071-1
Confidence scoreintact-miscore:0.56
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role APrey
Experimental role BBait
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:f4a
comment:"\"To investigate whether MR-1 regulates MEK/ERK signaling through physical interaction, a co-immunoprecipitation (Co-IP) assay was performed between MR-1, MEK1/2 and ERK1/2 in MCF7 cells. Immunoprecipitation with an antibody recognizing MR-1 showed that MEK1/2 and ERK1 co-precipitated with MR-1 (Fig. 4A), but ERK2 did not. Immunoprecipitation of endogenous MEK1/2, ERK1 and ERK2 also showed that MR-1 was bound to MEK1/2 and ERK1, but not ERK2 (Fig. 4A).\""
full coverage:Only protein-protein interactions
curation depth:imex curation
NCBI Taxonomy identifier for the host organismtaxid:9606(human-mcf7)
taxid:9606(Homo sapiens MCF-7 mammary adenocarcinoma cell)
Parameters of the interaction-
Creation date2014/07/16
Update date2014/10/16
negative Boolean valuefalse
Feature(s) for interactor A-
Feature(s) for interactor B-
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor AWestern blot
Participant identification method for interactor BWestern blot