Curation Details
Interaction ID: IM-21648-7

Unique identifier for interactor Auniprotkb:Q13526
Unique identifier for interactor Buniprotkb:Q13950
Alternative identifier for interactor Aintact:EBI-714158
Alternative identifier for interactor Bintact:EBI-976402
Aliases for Apsi-mi:pin1_human(display_long)
uniprotkb:Rotamase Pin1(gene name synonym)
uniprotkb:Peptidyl-prolyl cis-trans isomerase Pin1(gene name synonym)
uniprotkb:PIN1(gene name)
Aliases for Bpsi-mi:runx2_human(display_long)
uniprotkb:Oncogene AML-3(gene name synonym)
uniprotkb:Polyomavirus enhancer-binding protein 2 alpha A subunit(gene name synonym)
uniprotkb:SL3-3 enhancer factor 1 alpha A subunit(gene name synonym)
uniprotkb:SL3/AKV core-binding factor alpha A subunit(gene name synonym)
uniprotkb:Osteoblast-specific transcription factor 2(gene name synonym)
uniprotkb:Core-binding factor subunit alpha-1(gene name synonym)
uniprotkb:Acute myeloid leukemia 3 protein(gene name synonym)
uniprotkb:RUNX2(gene name)
uniprotkb:AML3(gene name synonym)
uniprotkb:CBFA1(gene name synonym)
uniprotkb:OSF2(gene name synonym)
uniprotkb:PEBP2A(gene name synonym)
Interaction detection methodspsi-mi:"MI:0096"(pull down)
First authorLee et al. (2013)
Identifier of the publicationimex:IM-21648
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0915"(physical association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-8772497
Confidence scoreintact-miscore:0.59
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role ABait
Experimental role BPrey
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:f3b f3d
comment:"\"We tested Ser or Tyr residue (including Ser16) substitution mutants of Pin1 (Fig. 3A) for the interaction with Runx2. Substitution of Ser16 to Ala abolished the binding of Pin1 to Runx2 (Fig. 3C). Tyr to Ala substitution (Y23A or Y24A) in the WW domain also significantly reduced the interaction with Runx2, whereas same substitution in the PPIase catalytic domain (Y94A) did not have any noticeable effect (Fig. 3D). Collectively, these results suggest that the WW domain of Pin1 is important for binding to Runx2\""
full coverage:Only protein-protein interactions
curation depth:imex curation
NCBI Taxonomy identifier for the host organismtaxid:-1(in vitro)
taxid:-1(In vitro)
Parameters of the interaction-
Creation date2013/09/25
Update date2014/10/16
negative Boolean valuefalse
Feature(s) for interactor Amutation decreasing interaction:23-23
glutathione s tranferase tag:?-?
Feature(s) for interactor Bbinding-associated region:92-513
myc tag:?-?
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor APredetermined participant
Participant identification method for interactor BAnti tag western blot