Curation Details
Interaction ID: IM-15683-4

Unique identifier for interactor Auniprotkb:P0AF26
Unique identifier for interactor Buniprotkb:P09152
Alternative identifier for interactor Aintact:EBI-555043
uniprotkb:P11351
Alternative identifier for interactor Bintact:EBI-547248
uniprotkb:P78294
Aliases for Apsi-mi:narj_ecoli(display_long)
uniprotkb:narJ(gene name)
psi-mi:narJ(display_short)
uniprotkb:b1226(locus name)
uniprotkb:JW1217(locus name)
uniprotkb:Redox enzyme maturation protein NarJ(gene name synonym)
Aliases for Bpsi-mi:narg_ecoli(display_long)
uniprotkb:narG(gene name)
psi-mi:narG(display_short)
uniprotkb:bisD(gene name synonym)
uniprotkb:narC(gene name synonym)
uniprotkb:b1224(locus name)
uniprotkb:Nitrate reductase A subunit alpha(gene name synonym)
uniprotkb:Quinol-nitrate oxidoreductase subunit alpha(gene name synonym)
uniprotkb:JW1215(locus name)
Interaction detection methodspsi-mi:"MI:0107"(surface plasmon resonance)
First authorZakian et al. (2010)
Identifier of the publicationpubmed:20236317
imex:IM-15683
mint:MINT-7557431
NCBI Taxonomy identifier for interactor Ataxid:83333(ecoli)
taxid:83333("Escherichia coli (strain K12)")
NCBI Taxonomy identifier for interactor Btaxid:83333(ecoli)
taxid:83333("Escherichia coli (strain K12)")
Interaction typespsi-mi:"MI:0407"(direct interaction)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-3893659
imex:IM-15683-4
Confidence scoreintact-miscore:0.93
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role ABait
Experimental role BPrey
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:none - see text under Fig 6
kinetics:"\"pH 7:-The results indicated the existence of a minor population (27%) with a high affinity (Kd= 4.4 +/- 3 × 10−9 m) and a major population (73%) with a lower affinity (Kd = 81 +/- 36 × 10−9 m). Analysis of the BIAcore experiments performed at pH 8 could only be fitted with the 1 : 1 Langmuir model of simple binding, confirming the existence of a single state of NarJ at this pH.\""
full coverage:Only protein-protein interactions
curation depth:imex curation
NCBI Taxonomy identifier for the host organismtaxid:-1(in vitro)
taxid:-1(In vitro)
Parameters of the interactionkd:4.4x10^-9(molar)
kd:81.0x10^-9(molar)
Creation date2010/02/04
Update date2024/11/14
negative Boolean valuefalse
Feature(s) for interactor Ahis tag:?-?
Feature(s) for interactor Bsufficient binding region:1-15
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor APredetermined participant
Participant identification method for interactor BPredetermined participant