Curation Details
Interaction ID: MINT-8308274

Unique identifier for interactor Auniprotkb:P84022
Unique identifier for interactor Buniprotkb:Q9HAU4
Alternative identifier for interactor Aintact:EBI-347161
uniprotkb:B7Z9Q2
uniprotkb:B7Z4Z5
uniprotkb:B7Z6M9
uniprotkb:F5H383
ensembl:ENSP00000332973.4
intact:EBI-1760241
uniprotkb:O09064
uniprotkb:O09144
uniprotkb:O14510
uniprotkb:O35273
uniprotkb:Q92940
uniprotkb:Q93002
uniprotkb:Q9GKR4
intact:EBI-1965323
uniprotkb:A8K4B6
Alternative identifier for interactor Bintact:EBI-396727
ensembl:ENSP00000262435.9
uniprotkb:Q52LL1
uniprotkb:Q9H260
Aliases for Apsi-mi:smad3_human(display_long)
uniprotkb:SMAD3(gene name)
psi-mi:SMAD3(display_short)
uniprotkb:MADH3(gene name synonym)
uniprotkb:SMAD family member 3(gene name synonym)
uniprotkb:JV15-2(gene name synonym)
Aliases for Bpsi-mi:smuf2_human(display_long)
uniprotkb:SMURF2(gene name)
psi-mi:SMURF2(display_short)
uniprotkb:SMAD ubiquitination regulatory factor 2(gene name synonym)
uniprotkb:SMAD-specific E3 ubiquitin-protein ligase 2(gene name synonym)
uniprotkb:HECT-type E3 ubiquitin transferase SMURF2(gene name synonym)
Interaction detection methodspsi-mi:"MI:0065"(isothermal titration calorimetry)
First authorTang et al. (2011)
Identifier of the publicationdoi:10.1038/emboj.2011.393
pubmed:22045334
imex:IM-16658
mint:MINT-8308197
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0407"(direct interaction)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-7500765
mint:MINT-8308274
imex:IM-16658-5
Confidence scoreintact-miscore:0.80
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role ANeutral component
Experimental role BNeutral component
Interactor type AProtein
Interactor type BProtein
Annotations for the interactioncomment:homomint
figure legend:Fig. 5c
comment:domino
comment:mint
kinetic_conditions:Temp = 25C
kinetics:"\"Smad3 peptide (173-189) phosphorylated at Thr179 binds to Smurf2 with Kd = 281 +/- 6 x 10E-9 molar. Unphosphorylated peptide binds much weaker, with Kd = 2971 +/- 158 x 10E-9 molar.\""
causality statement:Q9HAU4 decreases activity of P84022
causality statement:Q9HAU4 is required for ubiquitination of P84022
causality statement:Q9HAU4 decreases binding of P84022 to Q13485
causality statement:Q9HAU4 decreases binding of P84022 to P84022
dataset:IBD - Inflammatory bowel disease
full coverage:Only protein-protein interactions
curation depth:imex curation
NCBI Taxonomy identifier for the host organismtaxid:-1(in vitro)
taxid:-1(In vitro)
Parameters of the interactionkd:281.0x10^-9 ~6.0(molar)
Creation date2012/02/07
Update date2014/10/28
negative Boolean valuefalse
Feature(s) for interactor AO-phospho-L-threonine:179-179(MINT-8308281)
sufficient binding region:173-189(MINT-8308283)
Feature(s) for interactor Bsufficient binding region:248-367(MINT-8308294)
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor APeptide synthesis
Participant identification method for interactor BPredetermined participant