| Unique identifier for interactor A | uniprotkb:O15530 |
| Unique identifier for interactor B | uniprotkb:P27348 |
| Alternative identifier for interactor A | intact:EBI-717097 uniprotkb:Q8IV52 uniprotkb:Q9BRD5 uniprotkb:Q6FI20 uniprotkb:H0Y4Z0 uniprotkb:Q59EH6 ensembl:ENSP00000344220.4 intact:EBI-28968232 |
| Alternative identifier for interactor B | intact:EBI-359854 uniprotkb:Q567U5 uniprotkb:Q5TZU8 uniprotkb:Q9UP48 uniprotkb:D6W4Z5 ensembl:ENSP00000238081.3 ensembl:ENSP00000371267.4 |
| Aliases for A | psi-mi:pdpk1_human(display_long) uniprotkb:PDPK1(gene name) psi-mi:PDPK1(display_short) uniprotkb:PDK1(gene name synonym) |
| Aliases for B | psi-mi:1433t_human(display_long) uniprotkb:YWHAQ(gene name) psi-mi:YWHAQ(display_short) uniprotkb:14-3-3 protein tau(gene name synonym) uniprotkb:14-3-3 protein T-cell(gene name synonym) uniprotkb:Protein HS1(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0019"(coimmunoprecipitation) |
| First author | Sato et al. (2002) |
| Identifier of the publication | pubmed:12177059 mint:MINT-5215652 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0915"(physical association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-8509918 mint:MINT-16332 |
| Confidence score | intact-miscore:0.54 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Prey |
| Experimental role B | Bait |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | comment:"\"Co-IP 14-3-3-HA (pCRII vector Invitrogen) with PDK1-Myc (PCMV3 vector). Theta binds more tightlyrnC0-IP Endogenous: 14-3-3 Theta co-precipitates a fainth bando f PRK1 visible with anti PDK1. In 293T and NIH3T3rnGST pull down: GST-14-3-3 theta pulls down Myc- PDK1 and Raf from COS-7 lysatesrnCo-IP HA-14-3-3 theta with Flag-PDK1, if 14-3-3 is murarted R127A, less PDK1 is co-immunoprecipitated. 14-3-3 theta R56A,R60A binds as wt.rnCo-IP Flag-PDK1 with 14-3-3-HA is abolished if PDK1 has S241A: 238RANS241 or R238E. Binding increases if V243P (classical binding motif0.rnThe PRK1 site RSGS410 does not bind even if it is phosphorylated in vivo.rn\"" comment:homomint comment:mint dataset:Virus - Publications including interactions involving viral proteins |
| NCBI Taxonomy identifier for the host organism | taxid:-4(in vivo) taxid:-4(in vivo) |
| Parameters of the interaction | - |
| Creation date | 2003/01/16 |
| Update date | 2025/08/08 |
| negative Boolean value | false |
| Feature(s) for interactor A | binding-associated region:238-241(MINT-719228) |
| Feature(s) for interactor B | - |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Predetermined participant |
| Participant identification method for interactor B | Predetermined participant |