Curation Details
Interaction ID: IM-30502-7

Unique identifier for interactor Achebi:"CHEBI:63729"
Unique identifier for interactor Buniprotkb:P06730
Alternative identifier for interactor Aintact:EBI-67104107
Alternative identifier for interactor Bintact:EBI-73440
uniprotkb:Q96E95
uniprotkb:D6RCQ6
uniprotkb:B7Z6V1
ensembl:ENSP00000389624.2
intact:EBI-28969298
Aliases for Apsi-mi:7-methylguanosine 5'-diphosphate(display_short)
psi-mi:"CHEBI:63729"(display_long)
intact:7-methyl-GDP(synonym)
intact:"7-methylguanosine 5'-(dihydrogen diphosphate)"(synonym)
intact:7-methylguanosine 5'-diphosphate betaine(synonym)
intact:7-methylguanosine diphosphate(synonym)
intact:"5'-O-{hydroxy[(hydroxyphosphinato)oxy]phosphoryl}-7-methylguanosine"(iupac name)
Aliases for Bpsi-mi:if4e_human(display_long)
uniprotkb:mRNA cap-binding protein(gene name synonym)
uniprotkb:eIF-4F 25 kDa subunit(gene name synonym)
uniprotkb:EIF4E(gene name)
psi-mi:EIF4E(display_short)
uniprotkb:EIF4EL1(gene name synonym)
uniprotkb:EIF4F(gene name synonym)
Interaction detection methodspsi-mi:"MI:0077"(nuclear magnetic resonance)
First authorVolpon et al. (2017)
Identifier of the publicationpubmed:28325843
imex:IM-30502
NCBI Taxonomy identifier for interactor Ataxid:-2(chemical synthesis)
taxid:-2("Chemical synthesis (Chemical synthesis)")
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0407"(direct interaction)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-66898478
imex:IM-30502-7
Confidence scoreintact-miscore:0.62
Complex expansionpsi-mi:"MI:1060"(spoke expansion)
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role ANeutral component
Experimental role BNeutral component
Interactor type ASmall molecule
Interactor type BProtein
Annotations for the interactionfigure legend:fig. 1h,2
comment:"\"Using NMR methods, we observed that LRP10A associated with the eIF4E-m7 GDP cap complex (Fig. 2A–C) using the same surface as it used for cap-free eIF4E (Fig. 1J), including residues W73, H37, Q40, E70, N77, and G139. Importantly, cap-bound and cap-free eIF4E forms are readily distinguishable by HSQC, and we noted that eIF4E was still in the cap-bound form in the presence of LRP10A. Indeed, in the ternary complex m7 GDP–eIF4E–LRP10A, cross-peaks for residues close to or in contact with m7 GDP superimposed with those of the m7 GDP–eIF4E complex (Fig. 2D), indicating that LRP10A did not lead to substantial changes in the capbinding site of the cap–eIF4E complex\""
curation depth:imex curation
full coverage:Only protein-protein interactions
NCBI Taxonomy identifier for the host organismtaxid:-1(in vitro)
taxid:-1(In vitro)
Parameters of the interaction-
Creation date2025/07/10
Update date2025/12/09
negative Boolean valuefalse
Feature(s) for interactor A-
Feature(s) for interactor B15n label:?-?
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor APredetermined participant
Participant identification method for interactor BPredetermined participant