Curation Details
Interaction ID: IM-30212-19

Unique identifier for interactor Auniprotkb:O95714
Unique identifier for interactor Buniprotkb:P04049
Alternative identifier for interactor Aintact:EBI-1058922
uniprotkb:Q86SV7
uniprotkb:Q86SV8
uniprotkb:Q86SV9
uniprotkb:Q86YY3
uniprotkb:Q86YY4
uniprotkb:Q86YY5
uniprotkb:Q86YY6
uniprotkb:Q86YY7
uniprotkb:Q86YY8
uniprotkb:Q86YY9
uniprotkb:Q86YZ0
uniprotkb:Q86YZ1
intact:EBI-1104961
ensembl:ENSP00000261609.8
intact:EBI-28977852
Alternative identifier for interactor Bintact:EBI-365996
uniprotkb:B0LPH8
uniprotkb:Q15278
uniprotkb:Q9UC20
uniprotkb:B2R5N3
ensembl:ENSP00000251849.4
ensembl:ENSP00000508763.1
ensembl:ENSP00000509968.1
Aliases for Apsi-mi:herc2_human(display_long)
uniprotkb:HERC2(gene name)
psi-mi:HERC2(display_short)
uniprotkb:HECT domain and RCC1-like domain-containing protein 2(gene name synonym)
uniprotkb:HECT-type E3 ubiquitin transferase HERC2(gene name synonym)
Aliases for Bpsi-mi:raf1_human(display_long)
uniprotkb:RAF1(gene name)
psi-mi:RAF1(display_short)
uniprotkb:RAF(gene name synonym)
uniprotkb:Proto-oncogene c-RAF(gene name synonym)
uniprotkb:Raf-1(gene name synonym)
Interaction detection methodspsi-mi:"MI:0096"(pull down)
First authorSala-Gaston et al. (2022)
Identifier of the publicationpubmed:36241744
imex:IM-30212
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0915"(physical association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-61098419
imex:IM-30212-19
Confidence scoreintact-miscore:0.52
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role APrey
Experimental role BBait
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:suppl. fig.1d
comment:"\"In parallel, the same experiment was done but with the F5CT Myc-HERC2 construct instead of F4, and this revealed co-immunoprecipitation of F5CT with CR1 and CR3 (Supplementary Fig. 1D). constructs and performed a GFP pull-down, which showed preferential co-immunoprecipitation of the F4 construct with CR3 (Supplementary Fig. 1C).\""
curation depth:imex curation
full coverage:Only protein-protein interactions
NCBI Taxonomy identifier for the host organismtaxid:9606(human-293t)
taxid:9606(Homo sapiens HEK293T embryonic kidney cell)
Parameters of the interaction-
Creation date2024/11/05
Update date2025/07/31
negative Boolean valuefalse
Feature(s) for interactor Amyc tag:n-n
binding-associated region:4252-4834
Feature(s) for interactor Bgreen fluorescent protein tag:?-?
sufficient binding region:1-200,301-648
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor AAnti tag western blot
Participant identification method for interactor BAnti tag western blot