| Unique identifier for interactor A | uniprotkb:P04049 |
| Unique identifier for interactor B | uniprotkb:O95714 |
| Alternative identifier for interactor A | intact:EBI-365996 uniprotkb:B0LPH8 uniprotkb:Q15278 uniprotkb:Q9UC20 uniprotkb:B2R5N3 ensembl:ENSP00000251849.4 ensembl:ENSP00000508763.1 ensembl:ENSP00000509968.1 |
| Alternative identifier for interactor B | intact:EBI-1058922 uniprotkb:Q86SV7 uniprotkb:Q86SV8 uniprotkb:Q86SV9 uniprotkb:Q86YY3 uniprotkb:Q86YY4 uniprotkb:Q86YY5 uniprotkb:Q86YY6 uniprotkb:Q86YY7 uniprotkb:Q86YY8 uniprotkb:Q86YY9 uniprotkb:Q86YZ0 uniprotkb:Q86YZ1 intact:EBI-1104961 ensembl:ENSP00000261609.8 intact:EBI-28977852 |
| Aliases for A | psi-mi:raf1_human(display_long) uniprotkb:RAF1(gene name) psi-mi:RAF1(display_short) uniprotkb:RAF(gene name synonym) uniprotkb:Proto-oncogene c-RAF(gene name synonym) uniprotkb:Raf-1(gene name synonym) |
| Aliases for B | psi-mi:herc2_human(display_long) uniprotkb:HERC2(gene name) psi-mi:HERC2(display_short) uniprotkb:HECT domain and RCC1-like domain-containing protein 2(gene name synonym) uniprotkb:HECT-type E3 ubiquitin transferase HERC2(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0096"(pull down) |
| First author | Sala-Gaston et al. (2022) |
| Identifier of the publication | pubmed:36241744 imex:IM-30212 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0915"(physical association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-61098375 imex:IM-30212-13 |
| Confidence score | intact-miscore:0.52 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Bait |
| Experimental role B | Prey |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:suppl. fig.1a comment:"\"Constructs F4, F5, and F5CT coimmunoprecipitated with GFP-C-RAF, indicating that the HERC2 and C-RAF protein interaction occurs mainly in the carboxyl-terminus of HERC2 polypeptide chain. F5CT construct, which contains the HECT domain holding the ubiquitin ligase activity, showed the highest afnity with C-RAF, suggesting that this is the most relevant interaction site (Supplementary Fig. 1A)\"" curation depth:imex curation full coverage:Only protein-protein interactions |
| NCBI Taxonomy identifier for the host organism | taxid:9606(human-293t) taxid:9606(Homo sapiens HEK293T embryonic kidney cell) |
| Parameters of the interaction | - |
| Creation date | 2024/11/05 |
| Update date | 2025/07/31 |
| negative Boolean value | false |
| Feature(s) for interactor A | green fluorescent protein tag:?-? |
| Feature(s) for interactor B | sufficient binding region:2893-4834 myc tag:n-n |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Anti tag western blot |
| Participant identification method for interactor B | Anti tag western blot |