| Unique identifier for interactor A | uniprotkb:Q15034 |
| Unique identifier for interactor B | uniprotkb:P62750 |
| Alternative identifier for interactor A | intact:EBI-3906578 uniprotkb:A8K1S5 uniprotkb:Q8IXX3 ensembl:ENSP00000385684.1 |
| Alternative identifier for interactor B | intact:EBI-353254 uniprotkb:P29316 uniprotkb:Q92774 uniprotkb:P39024 uniprotkb:B2R5B2 ensembl:ENSP00000389103.2 |
| Aliases for A | psi-mi:herc3_human(display_long) uniprotkb:HERC3(gene name) psi-mi:HERC3(display_short) uniprotkb:KIAA0032(gene name synonym) uniprotkb:HECT domain and RCC1-like domain-containing protein 3(gene name synonym) uniprotkb:HECT-type E3 ubiquitin transferase HERC3(gene name synonym) |
| Aliases for B | psi-mi:rl23a_human(display_long) uniprotkb:RPL23A(gene name) psi-mi:RPL23A(display_short) uniprotkb:60S ribosomal protein L23a(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0997"(ubiquitinase assay) |
| First author | Zhang et al. (2021) |
| Identifier of the publication | imex:IM-30043 pubmed:35637966 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0220"(ubiquitination reaction) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-52354954 imex:IM-30043-15 |
| Confidence score | intact-miscore:0.66 |
| Complex expansion | - |
| Biological role A | Enzyme |
| Biological role B | Enzyme target |
| Experimental role A | Neutral component |
| Experimental role B | Neutral component |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:suppl. fig.7 - table 1 comment:"\"Mass spectrometry analysis showed that 11 Lys residues of RPL23A could be identified as possible side chains where HERC3 transmitted poly-Ub to (Supplementary Figure S7A-K, and Supplementary Table S1). Nine Lys residues with top scores were selected for further validation because these residues owned higher credible scores and were more reliable.Therefore, nine individual RPL23A mutants with K-to-R changes were constructed and subjected to in vivo ubiquitination experiments. The results indicated that HERC3-mediated ubiquitylation took place on K78, K89, and K123 of RPL23A (Figure 6C). As shown in Figure 6D, corresponding plasmids with wild-type HERC3 or several different deletion mutants were co-transfected into HEK293T.\"" curation depth:imex curation dataset:Cancer - Interactions investigated in the context of cancer full coverage:Only protein-protein interactions |
| NCBI Taxonomy identifier for the host organism | taxid:-1(in vitro) taxid:-1(In vitro) |
| Parameters of the interaction | - |
| Creation date | 2024/02/27 |
| Update date | 2025/08/07 |
| negative Boolean value | false |
| Feature(s) for interactor A | - |
| Feature(s) for interactor B | ubiquitinylated lysine:78-78 ubiquitinylated lysine:89-89 ubiquitinylated lysine:123-123 |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Sequence tag identification |
| Participant identification method for interactor B | Sequence tag identification |