| Unique identifier for interactor A | uniprotkb:Q2KHT3-1 |
| Unique identifier for interactor B | uniprotkb:P14373 |
| Alternative identifier for interactor A | intact:EBI-48447012 ensembl:ENSP00000387122.1 |
| Alternative identifier for interactor B | intact:EBI-719493 ensembl:ENSP00000366404.3 ensembl:ENSP00000383555.3 ensembl:ENSP00000392787.2 ensembl:ENSP00000405229.2 ensembl:ENSP00000414793.2 uniprotkb:A2BE15 uniprotkb:Q5RJA8 uniprotkb:Q5ST26 uniprotkb:Q6LA73 uniprotkb:Q6NXR9 uniprotkb:Q9BZY6 uniprotkb:Q9UJL3 |
| Aliases for A | psi-mi:q2kht3-1(display_long) uniprotkb:C-type lectin domain family 16 member A(gene name synonym) uniprotkb:CLEC16A(gene name) psi-mi:CLEC16A(display_short) uniprotkb:KIAA0350(gene name synonym) |
| Aliases for B | psi-mi:tri27_human(display_long) uniprotkb:TRIM27(gene name) psi-mi:TRIM27(display_short) uniprotkb:RNF76(gene name synonym) uniprotkb:RFP(gene name synonym) uniprotkb:Ret finger protein(gene name synonym) uniprotkb:Tripartite motif-containing protein 27(gene name synonym) uniprotkb:RING finger protein 76(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0007"(anti tag coimmunoprecipitation) |
| First author | Smits et al. (2023) |
| Identifier of the publication | pubmed:36538041 imex:IM-29939 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0915"(physical association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-48446734 imex:IM-29939-3 |
| Confidence score | intact-miscore:0.50 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Prey |
| Experimental role B | Bait |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:F3B comment:"\"To better understand the pathogenic effects of the variant identified in family 1 (p.Asn688Argfs*80), the deletion of exon 19 was introduced into the pEGFP-CLEC16A backbone by site-directed mutagenesis. Binding of pEGFP-CLEC16A-WT and -Δ19 to retromer complex component VPS35 was confirmed in an independent experiment after immunoprecipitation of pEGFP-CLEC16A-WT/Δ19 or GFP-only and detection of VPS35 on immunoblots (Fig. 3c and Supplementary Fig. 4c). Altogether, these analyses show that TRIM27 is a strong interactor of CLEC16A in HEK293T cells, an interaction that is lost by the human C-terminal truncated ∆19 variant.\"" dataset:Neurodevelopmental disease - Neurodevelopmental disorders are disabilities in the functioning of the brain that affect a child's behaviour, memory or ability to learn dataset:Rare diseases - Interactions investigated in the context of Rare genetic disease full coverage:Only protein-protein interactions curation depth:imex curation |
| NCBI Taxonomy identifier for the host organism | taxid:9606(human-293t) taxid:9606(Homo sapiens HEK293T embryonic kidney cell) |
| Parameters of the interaction | - |
| Creation date | 2023/10/26 |
| Update date | 2023/12/01 |
| negative Boolean value | false |
| Feature(s) for interactor A | green fluorescent protein tag:?-? necessary binding region:688-1053 |
| Feature(s) for interactor B | - |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Anti tag western blot |
| Participant identification method for interactor B | Anti tag western blot |