Curation Details
Interaction ID: IM-29508-5

Unique identifier for interactor Auniprotkb:Q9Z2E1
Unique identifier for interactor Buniprotkb:Q00566
Alternative identifier for interactor Aintact:EBI-5716946
uniprotkb:E9QMV9
uniprotkb:Q811D9
uniprotkb:Q9Z2D9
ensembl:ENSMUSP00000073701.5
Alternative identifier for interactor Bintact:EBI-9396907
uniprotkb:Q09HV7
uniprotkb:Q09HV8
Aliases for Apsi-mi:mbd2_mouse(display_long)
uniprotkb:Mbd2(gene name)
psi-mi:Mbd2(display_short)
uniprotkb:Methyl-CpG-binding protein MBD2(gene name synonym)
Aliases for Bpsi-mi:mecp2_rat(display_long)
uniprotkb:Mecp2(gene name)
psi-mi:Mecp2(display_short)
Interaction detection methodspsi-mi:"MI:0096"(pull down)
First authorBecker et al. (2012)
Identifier of the publicationimex:IM-29508
pubmed:23335972
NCBI Taxonomy identifier for interactor Ataxid:10090(mouse)
taxid:10090(Mus musculus)
NCBI Taxonomy identifier for interactor Btaxid:10116(rat)
taxid:10116("Rattus norvegicus (Rat)")
Interaction typespsi-mi:"MI:0407"(direct interaction)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-34185107
imex:IM-29508-5
Confidence scoreintact-miscore:0.57
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role ABait
Experimental role BPrey
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:f1d
comment:"\"The specific MeCP2 homo-interaction could also be detected upon addition of ethidium bromide (EtBr), underlining that the observed direct binding was independent from DNA bridging (Figure 1B). Furthermore, we could detect specific binding of MBD2G to immobilized RFP-fused MBD2 (MBD2R) (Figure 1C and Figure S1B) as well as of MeCP2G to immobilized MBD2R (Figure 1D and Figure S1B) without and upon addition of EtBr.\""
curation depth:imex curation
full coverage:Only protein-protein interactions
NCBI Taxonomy identifier for the host organismtaxid:-1(in vitro)
taxid:-1(In vitro)
Parameters of the interaction-
Creation date2022/03/11
Update date2022/06/01
negative Boolean valuefalse
Feature(s) for interactor Ared fluorescent protein tag:c-c
Feature(s) for interactor Bgreen fluorescent protein tag:?-?
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor AAnti tag western blot
Participant identification method for interactor BAnti tag western blot