Curation Details
Interaction ID: IM-28596-1

Unique identifier for interactor Auniprotkb:Q9HDC9
Unique identifier for interactor Bintact:EBI-26604519
Alternative identifier for interactor Aintact:EBI-723950
uniprotkb:Q6UVZ8
uniprotkb:Q9GZS8
uniprotkb:Q9NUB2
uniprotkb:A8K514
uniprotkb:B4DXG1
ensembl:ENSP00000217456.2
Alternative identifier for interactor B-
Aliases for Apsi-mi:apmap_human(display_long)
uniprotkb:Protein BSCv(gene name synonym)
uniprotkb:APMAP(gene name)
psi-mi:APMAP(display_short)
uniprotkb:C20orf3(gene name synonym)
uniprotkb:UNQ1869/PRO4305(orf name)
Aliases for Bpsi-mi:rtn4_crigr_protein(display_short)
psi-mi:EBI-26604519(display_long)
Interaction detection methodspsi-mi:"MI:0276"(blue native page)
First authorGerber et al. (2019)
Identifier of the publicationimex:IM-28596
pubmed:30704515
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:10029(crigr)
taxid:10029("Cricetulus griseus (Chinese hamster)")
Interaction typespsi-mi:"MI:0914"(association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-26573615
imex:IM-28596-1
Confidence scoreintact-miscore:0.35
Complex expansionpsi-mi:"MI:1060"(spoke expansion)
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role ABait
Experimental role BPrey
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:F3D SF4
comment:"\"We next designed a multistep purification procedure for the high-grade purification of APMAP and APMAP protein complexes. he Flag-tagged APMAP and APMAP protein complexes were affinity purified following a previously described M2 anti-Flag antibody immuno-affinity procedure [40]. As a last purification step, the proteins eluted from the M2 resin were separated by size exclusion chromatography (SEC) and the fractions enriched in APMAP (Fig. (Fig.3b)3b) were further loaded onto a Blue Native gel (BN-PAGE), which allows the detection of APMAP protein complexes in their native conformations.\""
curation depth:imex curation
dataset:Alzheimers - Interactions investigated in the context of Alzheimers disease
full coverage:Only protein-protein interactions
NCBI Taxonomy identifier for the host organismtaxid:10029(crigr-cho)
taxid:10029(chinese hamster ovary cell line)
Parameters of the interaction-
Creation date2020/12/28
Update date2024/11/14
negative Boolean valuefalse
Feature(s) for interactor Aflag tag:c-c
Feature(s) for interactor B-
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor ASequence tag identification
Participant identification method for interactor BSequence tag identification