| Unique identifier for interactor A | uniprotkb:Q9Y5P2 |
| Unique identifier for interactor B | uniprotkb:P04637 |
| Alternative identifier for interactor A | intact:EBI-26354757 uniprotkb:O75605 uniprotkb:Q0VDC3 uniprotkb:Q96IU8 ensembl:ENSP00000491011.1 |
| Alternative identifier for interactor B | intact:EBI-366083 uniprotkb:Q2XN98 uniprotkb:Q3LRW1 uniprotkb:Q3LRW2 uniprotkb:Q3LRW3 uniprotkb:Q3LRW4 uniprotkb:Q3LRW5 uniprotkb:Q15086 uniprotkb:Q15087 uniprotkb:Q15088 uniprotkb:Q16535 uniprotkb:Q16807 uniprotkb:Q16808 uniprotkb:Q16809 uniprotkb:Q16810 uniprotkb:Q16811 uniprotkb:Q16848 uniprotkb:Q86UG1 uniprotkb:Q8J016 uniprotkb:Q99659 uniprotkb:Q9BTM4 uniprotkb:Q9HAQ8 uniprotkb:Q9NP68 uniprotkb:Q9NPJ2 uniprotkb:Q9NZD0 uniprotkb:Q9UBI2 uniprotkb:Q9UQ61 ensembl:ENSP00000269305.4 ensembl:ENSP00000391478.2 ensembl:ENSP00000424104.2 ensembl:ENSP00000426252.2 intact:EBI-28980681 |
| Aliases for A | psi-mi:csag2_human(display_long) uniprotkb:Cancer/testis antigen 24.2(gene name synonym) uniprotkb:Taxol-resistant-associated gene 3 protein(gene name synonym) uniprotkb:CSAG2(gene name) psi-mi:CSAG2(display_short) uniprotkb:TRAG3(gene name synonym) uniprotkb:CSAG3(gene name) psi-mi:CSAG3(display_short) uniprotkb:CSAG3A(gene name synonym) |
| Aliases for B | psi-mi:p53_human(display_long) uniprotkb:TP53(gene name) psi-mi:TP53(display_short) uniprotkb:P53(gene name synonym) uniprotkb:Tumor suppressor p53(gene name synonym) uniprotkb:Phosphoprotein p53(gene name synonym) uniprotkb:Antigen NY-CO-13(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0415"(enzymatic study) |
| First author | Yang et al. (2020) |
| Identifier of the publication | pubmed:32761762 imex:IM-28361 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0197"(deacetylation reaction) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-26355472 imex:IM-28361-17 |
| Confidence score | intact-miscore:0.44 |
| Complex expansion | psi-mi:"MI:1060"(spoke expansion) |
| Biological role A | Stimulator |
| Biological role B | Enzyme target |
| Experimental role A | Neutral component |
| Experimental role B | Neutral component |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:fig. 6 e,f,g comment:"\"GST-CSAG2, but not GST alone, increased SIRT1-dependent p53 ac-K382 deacetylation in a dosedependent manner (Fig 6E). To investigate whether CSAG2- mediated p53 ac-K382 deacetylation requires interaction with SIRT1, in vitro SIRT1 activity assays were performed comparing CSAG2 wild-type and CSAG2 D37 mutant. In comparison with wild-type CSAG2, CSAG2 D37 failed to enhance SIRT1 enzymatic activity toward p53 ac-K382 (Fig 6F)\"" kinetics:"\"Surprisingly, SIRT1 Km for p53 ac-K382 peptide was not significantly different with or without CSAG2 (289 lM and 281 lM, respectively) (Fig 6G). However, CSAG2 significantly increased SIRT1 kcat from 8.3/s to 14.5/s (Fig 6G).\"" full coverage:Only protein-protein interactions curation depth:imex curation |
| NCBI Taxonomy identifier for the host organism | taxid:-1(in vitro) taxid:-1(In vitro) |
| Parameters of the interaction | - |
| Creation date | 2020/09/17 |
| Update date | 2020/09/22 |
| negative Boolean value | false |
| Feature(s) for interactor A | glutathione s tranferase tag:?-? binding-associated region:1-37 |
| Feature(s) for interactor B | binding-associated region:379-382 N-acetylated L-lysine:382-382 |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Predetermined participant |
| Participant identification method for interactor B | Tag visualisation by fluorescence |