Curation Details
Interaction ID: IM-28340-9

Unique identifier for interactor Auniprotkb:P49755
Unique identifier for interactor Buniprotkb:Q8XAL7
Alternative identifier for interactor Aintact:EBI-998422
uniprotkb:Q15602
uniprotkb:Q16536
uniprotkb:Q86TC2
uniprotkb:Q86TS5
uniprotkb:B2R605
intact:EBI-1058680
ensembl:ENSP00000303145.4
Alternative identifier for interactor Bintact:EBI-10039292
uniprotkb:Q7AEM9
Aliases for Apsi-mi:tmeda_human(display_long)
uniprotkb:21 kDa transmembrane-trafficking protein(gene name synonym)
uniprotkb:Transmembrane protein Tmp21(gene name synonym)
uniprotkb:Tmp-21-I(gene name synonym)
uniprotkb:p24delta(gene name synonym)
uniprotkb:S31III125(gene name synonym)
uniprotkb:TMED10(gene name)
psi-mi:TMED10(display_short)
uniprotkb:TMP21(gene name synonym)
uniprotkb:p23(gene name synonym)
uniprotkb:p24 family protein delta-1(gene name synonym)
uniprotkb:S31I125(gene name synonym)
Aliases for Bpsi-mi:nlef_eco57(display_long)
uniprotkb:nleF(gene name)
psi-mi:nleF(display_short)
uniprotkb:Non-LEE-encoded type III effector F(gene name synonym)
uniprotkb:Z6020.1(locus name)
uniprotkb:ECs1815(locus name)
Interaction detection methodspsi-mi:"MI:0007"(anti tag coimmunoprecipitation)
First authorOlsen et al. (2013)
Identifier of the publicationpubmed:23434013
imex:IM-28340
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:83334(eco57)
taxid:83334("Escherichia coli O157:H7")
Interaction typespsi-mi:"MI:0407"(direct interaction)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-25828337
imex:IM-28340-9
Confidence scoreintact-miscore:0.63
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role APrey
Experimental role BBait
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:fig.1b
comment:"\"By expressing and purifying recombinant forms of NleF and Tmp21 in E. coli BL21(DE3) and then using these proteins in pulldown assays, we confirmed that NleF and Tmp21 bind directly in vitro (Fig. 1B).\""
curation depth:imex curation
full coverage:Only protein-protein interactions
NCBI Taxonomy identifier for the host organismtaxid:-1(in vitro)
taxid:-1(In vitro)
Parameters of the interaction-
Creation date2020/09/08
Update date2024/11/14
negative Boolean valuefalse
Feature(s) for interactor Ahis tag:?-?
Feature(s) for interactor Bflag tag:?-?
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor AAnti tag western blot
Participant identification method for interactor BAnti tag western blot