Curation Details
Interaction ID: IM-28332-5

Unique identifier for interactor Auniprotkb:P55211
Unique identifier for interactor Buniprotkb:Q8XAL7
Alternative identifier for interactor Aintact:EBI-516799
ensembl:ENSP00000330237.5
uniprotkb:O95348
uniprotkb:Q92852
uniprotkb:Q9BQ62
uniprotkb:Q9UEQ3
uniprotkb:Q9UIJ8
uniprotkb:Q53Y70
uniprotkb:Q5JRU9
uniprotkb:Q5UGI1
uniprotkb:B4E1A3
Alternative identifier for interactor Bintact:EBI-10039292
uniprotkb:Q7AEM9
Aliases for Apsi-mi:casp9_human(display_long)
uniprotkb:ICE-like apoptotic protease 6(gene name synonym)
uniprotkb:Apoptotic protease Mch-6(gene name synonym)
uniprotkb:Apoptotic protease-activating factor 3(gene name synonym)
uniprotkb:CASP9(gene name)
psi-mi:CASP9(display_short)
uniprotkb:MCH6(gene name synonym)
Aliases for Bpsi-mi:nlef_eco57(display_long)
uniprotkb:nleF(gene name)
psi-mi:nleF(display_short)
uniprotkb:Non-LEE-encoded type III effector F(gene name synonym)
uniprotkb:Z6020.1(locus name)
uniprotkb:ECs1815(locus name)
Interaction detection methodspsi-mi:"MI:2318"(force measurement)
First authorBlasche et al. (2012)
Identifier of the publicationpubmed:23516580
imex:IM-28332
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:83334(eco57)
taxid:83334("Escherichia coli O157:H7")
Interaction typespsi-mi:"MI:0407"(direct interaction)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-25827349
imex:IM-28332-5
Confidence scoreintact-miscore:0.72
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role ABait
Experimental role BPrey
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:fig. 1c
comment:"\"The affinity constant (Kd) of NleF binding to immobilized caspase-9 as measured by surface acoustic wave (SAW) technology is ∼39 nM.\""
comment:"\"Binding kinetic and affinity was analysed on sam®5 BLUE and sam®5 GREEN instruments (SAW Instruments GmbH, Bonn, Germany). Both instruments use the \\"surface acoustic wave\\"-principle for label-free analysis of molecular binding in a flow-through system as described in Perpeet et al. (2006)\""
curation depth:imex curation
full coverage:Only protein-protein interactions
NCBI Taxonomy identifier for the host organismtaxid:-1(in vitro)
taxid:-1(In vitro)
Parameters of the interactionkd:39.0x10^-9(molar)
Creation date2020/09/08
Update date2020/09/11
negative Boolean valuefalse
Feature(s) for interactor A-
Feature(s) for interactor B-
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor APredetermined participant
Participant identification method for interactor BPredetermined participant