| Unique identifier for interactor A | uniprotkb:P62837 |
| Unique identifier for interactor B | uniprotkb:Q8X4X3 |
| Alternative identifier for interactor A | intact:EBI-347677 uniprotkb:Q3MN78 uniprotkb:D3DQC9 uniprotkb:Q96RP6 ensembl:ENSP00000381717.3 ensembl:ENSP00000381718.4 uniprotkb:P51669 |
| Alternative identifier for interactor B | intact:EBI-25438357 uniprotkb:A0A0H3JEU0 uniprotkb:Q7AE95 |
| Aliases for A | psi-mi:ub2d2_human(display_long) uniprotkb:UBC5B(gene name synonym) uniprotkb:UBCH4(gene name synonym) uniprotkb:UBE2D2(gene name) psi-mi:UBE2D2(display_short) uniprotkb:UBC4(gene name synonym) uniprotkb:UBCH5B(gene name synonym) uniprotkb:Ubiquitin-protein ligase D2(gene name synonym) uniprotkb:Ubiquitin carrier protein D2(gene name synonym) uniprotkb:Ubiquitin-conjugating enzyme E2-17 kDa 2(gene name synonym) uniprotkb:"Ubiquitin-conjugating enzyme E2(17)KB 2"(gene name synonym) uniprotkb:PUBC1(gene name synonym) uniprotkb:p53-regulated ubiquitin-conjugating enzyme 1(gene name synonym) uniprotkb:"(E3-independent) E2 ubiquitin-conjugating enzyme D2"(gene name synonym) uniprotkb:E2 ubiquitin-conjugating enzyme D2(gene name synonym) |
| Aliases for B | psi-mi:q8x4x3_eco57(display_long) uniprotkb:nleG5-1(gene name) psi-mi:nleG5-1(display_short) uniprotkb:ECs_1996(orf name) |
| Interaction detection methods | psi-mi:"MI:0053"(fluorescence polarization spectroscopy) |
| First author | Wu et al. (2010) |
| Identifier of the publication | pubmed:20585566 imex:IM-27765 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:83334(eco57) taxid:83334("Escherichia coli O157:H7") |
| Interaction types | psi-mi:"MI:0407"(direct interaction) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-25438319 imex:IM-27765-3 |
| Confidence score | intact-miscore:0.44 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Neutral component |
| Experimental role B | Neutral component |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:fig. 7c comment:"\"To quantify the NleG5-1[1–213] interactions with UBE2D2 variants, we labelled the three UBE2D2 mutant proteins described above with fluorescein-5-maleimide and monitored the change in fluorescence polarization upon titration of NleG5-1[1–213] (Figure 7C). Kd values for NleG5-1[1–213] interactions with UBE2D2 R5A, F62A and K63A mutants were 326±35, 36±2 and 213±9 µM, respectively. Compared to the Kd for NleG5-1[1–213] interaction with wild type UBE2D2, presented above (56±2 µM) the affinity of R5A and K63A mutants were approximately 5 and 3 times lower, respectively, while the affinity of the F62A mutant was slightly higher.\"" full coverage:Only protein-protein interactions curation depth:imex curation |
| NCBI Taxonomy identifier for the host organism | taxid:-1(in vitro) taxid:-1(In vitro) |
| Parameters of the interaction | kd:56.0x10^-6 ~2.0(molar) |
| Creation date | 2020/02/24 |
| Update date | 2020/03/02 |
| negative Boolean value | false |
| Feature(s) for interactor A | mutation increasing interaction rate:62-62 fluorescein-5-maleimide label:?-? mutation decreasing interaction rate:5-5 mutation decreasing interaction rate:63-63 |
| Feature(s) for interactor B | - |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Tag visualisation by fluorescence |
| Participant identification method for interactor B | Tag visualisation by fluorescence |