| Unique identifier for interactor A | uniprotkb:Q8X834 |
| Unique identifier for interactor B | uniprotkb:Q04206 |
| Alternative identifier for interactor A | intact:EBI-10039193 uniprotkb:A0A6M0JJK4 uniprotkb:A0A7U8R4U9 uniprotkb:Q7AGE2 uniprotkb:A0A6M6W2Q6 |
| Alternative identifier for interactor B | intact:EBI-73886 ensembl:ENSP00000384273.3 uniprotkb:Q6GTV1 uniprotkb:Q6SLK1 |
| Aliases for A | psi-mi:q8x834_eco57(display_long) uniprotkb:nleC(gene name) psi-mi:nleC(display_short) uniprotkb:ECs_0847(orf name) |
| Aliases for B | psi-mi:tf65_human(display_long) uniprotkb:Nuclear factor NF-kappa-B p65 subunit(gene name synonym) uniprotkb:RELA(gene name) psi-mi:RELA(display_short) uniprotkb:NFKB3(gene name synonym) uniprotkb:Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0435"(protease assay) |
| First author | Yen et al. (2009) |
| Identifier of the publication | pubmed:21187904 imex:IM-27757 |
| NCBI Taxonomy identifier for interactor A | taxid:83334(eco57) taxid:83334("Escherichia coli O157:H7") |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0194"(cleavage reaction) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-25463697 imex:IM-27757-1 |
| Confidence score | intact-miscore:0.59 |
| Complex expansion | - |
| Biological role A | Enzyme |
| Biological role B | Enzyme target |
| Experimental role A | Neutral component |
| Experimental role B | Neutral component |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:fig.4b, 4d comment:"\"Next, to determine the dependency of this cleavage on host cofactors, purified p65 was mixed with GST-NleC. While both the p65 only and GST-added control samples showed no change in total p65 nor the detection of p65-C, the GST-NleC-containing mixture had less p65, and p65-C was readily detected (Fig. 4B). Based on these observations, we concluded that NleC could mediate the direct digestion of p65.\"" antagonist:"\"EDTA: CHEBI:64758\"" full coverage:Only protein-protein interactions curation depth:imex curation |
| NCBI Taxonomy identifier for the host organism | taxid:-1(in vitro) taxid:-1(In vitro) |
| Parameters of the interaction | - |
| Creation date | 2020/03/03 |
| Update date | 2020/03/03 |
| negative Boolean value | false |
| Feature(s) for interactor A | mutation disrupting interaction:196-196 glutathione s tranferase tag:?-? |
| Feature(s) for interactor B | - |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Western blot |
| Participant identification method for interactor B | Western blot |