Curation Details
Interaction ID: IM-27748-20

Unique identifier for interactor Auniprotkb:Q15599
Unique identifier for interactor Buniprotkb:B7UR60
Alternative identifier for interactor Aintact:EBI-1149760
uniprotkb:D3DU84
uniprotkb:D3DU85
uniprotkb:H3BSV6
intact:EBI-15579694
ensembl:ENSP00000408005.2
uniprotkb:O00272
uniprotkb:O00556
uniprotkb:Q3KQY7
Alternative identifier for interactor Bintact:EBI-25435618
Aliases for Apsi-mi:nhrf2_human(display_long)
uniprotkb:NHERF2(gene name)
psi-mi:NHERF2(display_short)
uniprotkb:NHE3 kinase A regulatory protein E3KARP(gene name synonym)
uniprotkb:SLC9A3R2(gene name synonym)
uniprotkb:SRY-interacting protein 1(gene name synonym)
uniprotkb:Sodium-hydrogen exchanger regulatory factor 2(gene name synonym)
uniprotkb:Solute carrier family 9 isoform A3 regulatory factor 2(gene name synonym)
uniprotkb:Tyrosine kinase activator protein 1(gene name synonym)
Aliases for Bpsi-mi:b7ur60_eco27(display_long)
uniprotkb:E2348C_1442(locus name)
Interaction detection methodspsi-mi:"MI:0007"(anti tag coimmunoprecipitation)
First authorMartinez et al. (2010)
Identifier of the publicationpubmed:20618342
imex:IM-27748
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:574521(eco27)
taxid:574521("Escherichia coli O127:H6 (strain E2348/69 / EPEC)")
Interaction typespsi-mi:"MI:0915"(physical association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-25436623
imex:IM-27748-20
Confidence scoreintact-miscore:0.69
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role ABait
Experimental role BPrey
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:fig.2a
comment:"\"Probing infected cell extracts with anti-EspI and anti NleH1 revealed that while both full-length EspI and EspIΔC7 were found in equal amounts, NleH1ΔC4 was unstable (Fig. 2A and B). Following cell lysis the supernatants were subjected to co-immunoprecipitation with anti-HA antibodies. While NHERF2 did not co-immunoprecipitate EspIΔC7 (Fig. 2A) and NleH1ΔC4 (Fig. 2B), both full-length EspI (Fig. 2A) and NleH1 (Fig. 2B) were co-immunoprecipitated.\""
full coverage:Only protein-protein interactions
curation depth:imex curation
NCBI Taxonomy identifier for the host organismtaxid:9606(human-hela)
taxid:9606(Homo sapiens HeLa epitheloid cervical carcinoma cell)
Parameters of the interaction-
Creation date2020/02/24
Update date2020/02/26
negative Boolean valuefalse
Feature(s) for interactor Aha tag:?-?
Feature(s) for interactor B-
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor AWestern blot
Participant identification method for interactor BWestern blot