| Unique identifier for interactor A | uniprotkb:Q9NTX7 |
| Unique identifier for interactor B | uniprotkb:Q9NTX7 |
| Alternative identifier for interactor A | intact:EBI-722397 uniprotkb:Q6FIB2 uniprotkb:Q7L8H4 uniprotkb:Q96K03 uniprotkb:Q9NTX6 uniprotkb:E1P572 uniprotkb:Q96T06 ensembl:ENSP00000357297.1 ensembl:ENSP00000476814.1 intact:EBI-28979460 |
| Alternative identifier for interactor B | intact:EBI-722397 uniprotkb:Q6FIB2 uniprotkb:Q7L8H4 uniprotkb:Q96K03 uniprotkb:Q9NTX6 uniprotkb:E1P572 uniprotkb:Q96T06 ensembl:ENSP00000357297.1 ensembl:ENSP00000476814.1 intact:EBI-28979460 |
| Aliases for A | psi-mi:rn146_human(display_long) uniprotkb:Dactylidin(gene name synonym) uniprotkb:RNF146(gene name) psi-mi:RNF146(display_short) uniprotkb:RING finger protein 146(gene name synonym) uniprotkb:Iduna(gene name synonym) uniprotkb:RING-type E3 ubiquitin transferase RNF146(gene name synonym) |
| Aliases for B | psi-mi:rn146_human(display_long) uniprotkb:Dactylidin(gene name synonym) uniprotkb:RNF146(gene name) psi-mi:RNF146(display_short) uniprotkb:RING finger protein 146(gene name synonym) uniprotkb:Iduna(gene name synonym) uniprotkb:RING-type E3 ubiquitin transferase RNF146(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0007"(anti tag coimmunoprecipitation) |
| First author | Callow et al. (2010) |
| Identifier of the publication | pubmed:21799911 imex:IM-27448 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0914"(association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-22117202 imex:IM-27448-1 |
| Confidence score | intact-miscore:0.35 |
| Complex expansion | psi-mi:"MI:1060"(spoke expansion) |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Bait |
| Experimental role B | Prey |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:fig. 5c 5d comment:"\"s a complimentary approach to identify RNF146 interacting proteins, we expressed in HEK293 cells RNF146 wildtype and ΔRING proteins, as well the control E3 ligase HECTD1, and immunoprecipitated these HA-tagged proteins from cell lysates. Mass spectrometric identification of proteins in all Coomassie-stained electrophoretic gel bands (Figure 5C) yielded TNKS1, TNKS2, PARP1, and PARP2 among the most prevalent proteins captured by RNF146ΔRING but not HECTD1, in addition to 11 other proteins known to bind PARP1/2 or function in DNA damage response (Figure 5D). Most of these protein interactors were also captured by wildtype RNF146 protein, although typically identified by fewer total peptides, suggesting that ubiquitylation by active RNF146 may release interactors from a complex. In contrast, specific interacting proteins that were identified by more peptides after capture by wildtype rather than ΔRING RNF146 include ubiquitin and the HECT-type ubiquitin E3 ligase HUWE1.\"" curation depth:imex curation full coverage:Only protein-protein interactions |
| NCBI Taxonomy identifier for the host organism | taxid:9606(human-293) taxid:9606(Homo sapiens HEK293 embryonic kidney cell) |
| Parameters of the interaction | - |
| Creation date | 2019/10/22 |
| Update date | 2024/09/03 |
| negative Boolean value | false |
| Feature(s) for interactor A | ha tag:?-? |
| Feature(s) for interactor B | - |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Sequence tag identification |
| Participant identification method for interactor B | Sequence tag identification |