Curation Details
Interaction ID: IM-27434-3

Unique identifier for interactor Auniprotkb:Q02156
Unique identifier for interactor Buniprotkb:P15056
Alternative identifier for interactor Aintact:EBI-706254
uniprotkb:B0LPH7
ensembl:ENSP00000306124.3
uniprotkb:Q32MQ3
uniprotkb:Q53SL4
uniprotkb:Q53SM5
uniprotkb:Q9UE81
Alternative identifier for interactor Bintact:EBI-365980
uniprotkb:A4D1T4
uniprotkb:B6HY61
uniprotkb:B6HY62
uniprotkb:B6HY63
uniprotkb:B6HY64
uniprotkb:B6HY65
uniprotkb:B6HY66
uniprotkb:Q13878
uniprotkb:Q3MIN6
ensembl:ENSP00000493543.1
intact:EBI-28988924
uniprotkb:Q9UDP8
uniprotkb:Q9Y6T3
Aliases for Apsi-mi:kpce_human(display_long)
uniprotkb:nPKC-epsilon(gene name synonym)
uniprotkb:PRKCE(gene name)
psi-mi:PRKCE(display_short)
uniprotkb:PKCE(gene name synonym)
Aliases for Bpsi-mi:braf_human(display_long)
uniprotkb:BRAF(gene name)
psi-mi:BRAF(display_short)
uniprotkb:BRAF1(gene name synonym)
uniprotkb:RAFB1(gene name synonym)
uniprotkb:v-Raf murine sarcoma viral oncogene homolog B1(gene name synonym)
uniprotkb:p94(gene name synonym)
uniprotkb:Proto-oncogene B-Raf(gene name synonym)
Interaction detection methodspsi-mi:"MI:0006"(anti bait coimmunoprecipitation)
First authorCarmo et al. (2010)
Identifier of the publicationpubmed:21625473
imex:IM-27434
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0915"(physical association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-22091416
imex:IM-27434-3
Confidence scoreintact-miscore:0.59
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role ABait
Experimental role BPrey
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:fig. 2b
comment:"\"To substantiate the notion that the three proteins interacted, we next immunoprecipitated either PKCĪµ (Fig. 2B) or B-RAF (Fig. 2C) from cell lysates. We then performed western blotting to probe for these proteins and S6K2. While B-RAF and PKCĪµ basal interaction increased 10 min after FGF-2 stimulation and subsequently persisted, we were unable to detect S6K2 in these immunoprecipitates (Fig. 2B, 2C and data not shown).\""
variable_condition:FGF2 treatment increases interaction at 10'
full coverage:Only protein-protein interactions
curation depth:imex curation
NCBI Taxonomy identifier for the host organismtaxid:9606(human-u2os)
taxid:9606(Homo sapiens U2OS osteosarcoma cell)
Parameters of the interaction-
Creation date2019/10/14
Update date2019/10/16
negative Boolean valuefalse
Feature(s) for interactor A-
Feature(s) for interactor B-
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor AWestern blot
Participant identification method for interactor BWestern blot