| Unique identifier for interactor A | uniprotkb:O15037 |
| Unique identifier for interactor B | uniprotkb:Q15843 |
| Alternative identifier for interactor A | intact:EBI-6148525 uniprotkb:Q86TZ6 uniprotkb:Q8IUQ2 uniprotkb:Q96BA9 ensembl:ENSP00000251343.5 ensembl:ENSP00000450799.1 ensembl:ENSP00000451106.1 |
| Alternative identifier for interactor B | intact:EBI-716247 uniprotkb:Q3SXN8 uniprotkb:Q6LES6 ensembl:ENSP00000250495.5 ensembl:ENSP00000496420.1 |
| Aliases for A | psi-mi:khnyn_human(display_long) uniprotkb:KHNYN(gene name) psi-mi:KHNYN(display_short) uniprotkb:KH and NYN domain-containing protein(gene name synonym) uniprotkb:KIAA0323(gene name synonym) |
| Aliases for B | psi-mi:nedd8_human(display_long) uniprotkb:Ubiquitin-like protein Nedd8(gene name synonym) uniprotkb:Neddylin(gene name synonym) uniprotkb:Neural precursor cell expressed developmentally down-regulated protein 8(gene name synonym) uniprotkb:NEDD8(gene name) psi-mi:NEDD8(display_short) |
| Interaction detection methods | psi-mi:"MI:0096"(pull down) |
| First author | Castagnoli et al. (2019) |
| Identifier of the publication | pubmed:30659753 imex:IM-26819 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0915"(physical association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-21221404 imex:IM-26819-9 |
| Confidence score | intact-miscore:0.70 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Bait |
| Experimental role B | Prey |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:fig 3c, fig 5b comment:"\"T‐Rex‐flag‐NEDD8 cell lysates were therefore incubated with the purified proteins bound to glutathione‐Sepharose beads and affinity‐purified proteins were analysed by western blotting with anti‐flag antibody (Fig. 3C). As observed, the purified UBDs considerably differ in their ability to bind neddylated substrates, KHNYN being the one displaying the highest binding efficiency. In addition, the UBD of KHNYN was the only one able to bind both neddylated substrates and unconjugated flag‐NEDD8, as detected by longer exposure.\"" comment:"\"To further confirm that the molecular determinants governing NEDD8 recognition are found in the C‐terminal 52 residues of KHNYN, we generated two chimeras by swapping the amino acid regions corresponding to the three α‐helices. The first chimeric construct, KN, contains the N‐terminal unique region of KHNYN (aa 598–634) and the α‐helices of N4BP1 (aa 854–896), while in the NK construct the last three helices of KHNYN (aa 635–678) are fused to the N‐terminal region of N4BP1 (aa 813–852) (Fig. 5C). The purified recombinant proteins were analysed by pull‐down assays for the ability to bind neddylated substrates in the T‐Rex‐flag‐NEDD8 cells. As shown, the chimeric construct NK retains the properties of wild‐type KHNYN, as it is able to bind to conjugated and monomeric NEDD8.\"" curation depth:imex curation full coverage:Only protein-protein interactions |
| NCBI Taxonomy identifier for the host organism | taxid:-1(in vitro) taxid:-1(In vitro) |
| Parameters of the interaction | - |
| Creation date | 2019/02/19 |
| Update date | 2024/11/14 |
| negative Boolean value | false |
| Feature(s) for interactor A | sufficient binding region:627-678 glutathione s tranferase tag:?-? |
| Feature(s) for interactor B | flag tag:?-? |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Western blot |
| Participant identification method for interactor B | Western blot |