Curation Details
Interaction ID: IM-26819-77

Unique identifier for interactor Auniprotkb:O15037
Unique identifier for interactor Buniprotkb:Q15843
Alternative identifier for interactor Aintact:EBI-6148525
uniprotkb:Q86TZ6
uniprotkb:Q8IUQ2
uniprotkb:Q96BA9
ensembl:ENSP00000251343.5
ensembl:ENSP00000450799.1
ensembl:ENSP00000451106.1
Alternative identifier for interactor Bintact:EBI-716247
uniprotkb:Q3SXN8
uniprotkb:Q6LES6
ensembl:ENSP00000250495.5
ensembl:ENSP00000496420.1
Aliases for Apsi-mi:khnyn_human(display_long)
uniprotkb:KHNYN(gene name)
psi-mi:KHNYN(display_short)
uniprotkb:KH and NYN domain-containing protein(gene name synonym)
uniprotkb:KIAA0323(gene name synonym)
Aliases for Bpsi-mi:nedd8_human(display_long)
uniprotkb:Ubiquitin-like protein Nedd8(gene name synonym)
uniprotkb:Neddylin(gene name synonym)
uniprotkb:Neural precursor cell expressed developmentally down-regulated protein 8(gene name synonym)
uniprotkb:NEDD8(gene name)
psi-mi:NEDD8(display_short)
Interaction detection methodspsi-mi:"MI:0096"(pull down)
First authorCastagnoli et al. (2019)
Identifier of the publicationpubmed:30659753
imex:IM-26819
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0407"(direct interaction)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-21222635
imex:IM-26819-77
Confidence scoreintact-miscore:0.70
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role APrey
Experimental role BBait
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:fig 11b
comment:"\"To confirm our model, we mutated residues Glu31 and Glu32 in NEDD8 by substituting them with the corresponding residues in ubiquitin (Glu31Gln and Glu32Asp) or with an opposite charge (Glu31Lys and Glu32Lys). These mutants, together with the Ile44Ala and the Ala72Arg mutants, were assayed in a pull‐down experiment against the purified CUBAN domain (Fig. 11B). As shown, there is a clear loss of binding when mutating the hydrophobic patch key residue Ile44, while the Ala72Arg mutation reduces the binding efficiency as previously shown. In addition, there is a reduction in binding efficiency when using the mutants H25F and R38E, but not K26D and R33E, in the electrostatic binding surface of CUBAN domain (Fig. 11C).\""
curation depth:imex curation
full coverage:Only protein-protein interactions
NCBI Taxonomy identifier for the host organismtaxid:-1(in vitro)
taxid:-1(In vitro)
Parameters of the interaction-
Creation date2019/02/19
Update date2024/11/14
negative Boolean valuefalse
Feature(s) for interactor Abinding-associated region:598-678
Feature(s) for interactor Bglutathione s tranferase tag:?-?
mutation increasing interaction:31-31
mutation disrupting interaction:44-44
mutation decreasing interaction:72-72
mutation decreasing interaction:31-32
mutation decreasing interaction:31-31
mutation decreasing interaction:31-31
mutation decreasing interaction:32-32
mutation disrupting interaction:31-32
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor AWestern blot
Participant identification method for interactor BWestern blot