| Unique identifier for interactor A | uniprotkb:O15037 |
| Unique identifier for interactor B | uniprotkb:Q15843 |
| Alternative identifier for interactor A | intact:EBI-6148525 uniprotkb:Q86TZ6 uniprotkb:Q8IUQ2 uniprotkb:Q96BA9 ensembl:ENSP00000251343.5 ensembl:ENSP00000450799.1 ensembl:ENSP00000451106.1 |
| Alternative identifier for interactor B | intact:EBI-716247 ensembl:ENSP00000250495.5 ensembl:ENSP00000496420.1 uniprotkb:Q3SXN8 uniprotkb:Q6LES6 |
| Aliases for A | psi-mi:khnyn_human(display_long) uniprotkb:KHNYN(gene name) psi-mi:KHNYN(display_short) uniprotkb:KH and NYN domain-containing protein(gene name synonym) uniprotkb:KIAA0323(gene name synonym) |
| Aliases for B | psi-mi:nedd8_human(display_long) uniprotkb:Ubiquitin-like protein Nedd8(gene name synonym) uniprotkb:Neddylin(gene name synonym) uniprotkb:Neural precursor cell expressed developmentally down-regulated protein 8(gene name synonym) uniprotkb:NEDD8(gene name) psi-mi:NEDD8(display_short) |
| Interaction detection methods | psi-mi:"MI:0065"(isothermal titration calorimetry) |
| First author | Castagnoli et al. (2019) |
| Identifier of the publication | pubmed:30659753 imex:IM-26819 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0407"(direct interaction) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-21222588 imex:IM-26819-59 |
| Confidence score | intact-miscore:0.70 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Neutral component |
| Experimental role B | Neutral component |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:fig 7b comment:"\"ITC titration of NEDD8 with the KHNYN domain shows an exothermic binding thermogram (Fig. 7B). The uncertainty in the early steps of the exponential curve allows only rough indications of the binding affinity of the CUBAN domain of KHNYN in complex with NEDD8 that was evaluated to be around 24 ± 2 μm. Finally, NEDD8 A72R mutation causes a reduction in the interaction efficiency (44 ± 7 μm) while binding of KHNYN for ubiquitin was nearly undetectable in these conditions.\"" full coverage:Only protein-protein interactions curation depth:imex curation |
| NCBI Taxonomy identifier for the host organism | taxid:-1(in vitro) taxid:-1(In vitro) |
| Parameters of the interaction | kd:24.0x10^-6 ~2.0(molar) |
| Creation date | 2019/02/19 |
| Update date | 2019/02/22 |
| negative Boolean value | false |
| Feature(s) for interactor A | binding-associated region:598-678 |
| Feature(s) for interactor B | mutation decreasing interaction:72-72 |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Predetermined participant |
| Participant identification method for interactor B | Predetermined participant |