Curation Details
Interaction ID: IM-26634-1

Unique identifier for interactor Auniprotkb:Q99816
Unique identifier for interactor Bensembl:ENSG00000124762
Alternative identifier for interactor Aintact:EBI-346882
uniprotkb:Q9BUM5
ensembl:ENSP00000251968.3
Alternative identifier for interactor Bintact:EBI-1550784
Aliases for Apsi-mi:ts101_human(display_long)
uniprotkb:TSG101(gene name)
psi-mi:TSG101(display_short)
uniprotkb:ESCRT-I complex subunit TSG101(gene name synonym)
Aliases for Bpsi-mi:cdkn1a_human_gene(display_short)
psi-mi:ENSG00000124762(display_long)
Interaction detection methodspsi-mi:"MI:0402"(chromatin immunoprecipitation assay)
First authorLin et al. (2012)
Identifier of the publicationimex:IM-26634
pubmed:24244542
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0915"(physical association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-20621815
imex:IM-26634-1
Confidence scoreintact-miscore:0.40
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role ABait
Experimental role BPrey
Interactor type AProtein
Interactor type BGene
Annotations for the interactionfigure legend:f3a f4b
comment:"\"Chromatin immunoprecipitation (ChIP) assays showed that sequences of the p21 promoter region interact directly with the TSG101 protein and identified the TSG101-binding sequence in Saos-2 as the region amplified by primer set ā€œdā€ (Fig 3A). However, failure of a construct containing only the UEV domain to bind indicates that at the presence of either the PR or CC domain enables binding. Collectively these findings indicate that TSG101 contains more than one locus that allows it to interact with the p21 promoter.\""
causality statement:Q99816 activates ENSG00000124762
causality statement:down-regulates activity
curation depth:imex curation
full coverage:Only protein-protein interactions
NCBI Taxonomy identifier for the host organismtaxid:9606(human-saos-2)
taxid:9606(Homo sapiens SAOS-2 osteosarcoma cell)
Parameters of the interaction-
Creation date2018/07/12
Update date2024/11/14
negative Boolean valuefalse
Feature(s) for interactor Anecessary binding region:312-390
Feature(s) for interactor Bsufficient binding region:?-?
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor APrimer specific pcr
Participant identification method for interactor BPrimer specific pcr