Curation Details
Interaction ID: IM-26539-31

Unique identifier for interactor Auniprotkb:P30556
Unique identifier for interactor Buniprotkb:O75937
Alternative identifier for interactor Aintact:EBI-6623016
uniprotkb:Q8TBK4
uniprotkb:Q13725
ensembl:ENSP00000273430.3
ensembl:ENSP00000385612.2
ensembl:ENSP00000385641.3
ensembl:ENSP00000398832.4
ensembl:ENSP00000418084.1
ensembl:ENSP00000418851.1
ensembl:ENSP00000419422.1
ensembl:ENSP00000419783.1
Alternative identifier for interactor Bintact:EBI-1045911
uniprotkb:Q6IBA4
uniprotkb:Q8N4Z5
uniprotkb:Q9P051
uniprotkb:Q9P067
uniprotkb:D3DPM0
uniprotkb:B4DUU4
intact:EBI-28966785
ensembl:ENSP00000263697.4
Aliases for Apsi-mi:agtr1_human(display_long)
uniprotkb:AGTR1(gene name)
psi-mi:AGTR1(display_short)
uniprotkb:AGTR1A(gene name synonym)
uniprotkb:AGTR1B(gene name synonym)
uniprotkb:AT2R1(gene name synonym)
uniprotkb:AT2R1B(gene name synonym)
uniprotkb:AT1AR(gene name synonym)
uniprotkb:AT1BR(gene name synonym)
uniprotkb:Angiotensin II type-1 receptor(gene name synonym)
Aliases for Bpsi-mi:dnjc8_human(display_long)
uniprotkb:Splicing protein spf31(gene name synonym)
uniprotkb:DNAJC8(gene name)
psi-mi:DNAJC8(display_short)
uniprotkb:SPF31(gene name synonym)
uniprotkb:HSPC315(orf name)
uniprotkb:HSPC331(orf name)
Interaction detection methodspsi-mi:"MI:0007"(anti tag coimmunoprecipitation)
First authorSokolina et al. (2017)
Identifier of the publicationpubmed:28298427
doi:10.15252/msb.20167430
imex:IM-26539
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0915"(physical association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-20796679
imex:IM-26539-31
Confidence scoreintact-miscore:0.51
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role ABait
Experimental role BPrey
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:F4A Figure EV4 Table EV6
comment:In the first approach, FLAG‐tagged GPCR interactors were overexpressed in mammalian cells, pulled‐down using anti‐FLAG antibody, subjected to SDS–PAGE, transferred to membranes, and probed with commercial antibody raised against their identified endogenous GPCR‐interaction partner.
curation depth:imex curation
full coverage:Only protein-protein interactions
NCBI Taxonomy identifier for the host organismtaxid:9606(human-293t)
taxid:9606(Homo sapiens HEK293T embryonic kidney cell)
Parameters of the interaction-
Creation date2018/07/06
Update date2024/11/14
negative Boolean valuefalse
Feature(s) for interactor Aflag tag:?-?
Feature(s) for interactor B-
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor AWestern blot
Participant identification method for interactor BWestern blot