Curation Details
Interaction ID: IM-26539-25

Unique identifier for interactor Auniprotkb:P35372
Unique identifier for interactor Buniprotkb:Q9H3P2
Alternative identifier for interactor Aintact:EBI-2624570
uniprotkb:G8XRH8
uniprotkb:B2R9S7
uniprotkb:B8Q1L7
uniprotkb:Q12930
uniprotkb:Q4VWM1
uniprotkb:Q4VWM2
uniprotkb:Q4VWM3
uniprotkb:Q4VWM4
uniprotkb:Q4VWM6
uniprotkb:Q4VWX6
uniprotkb:Q5TDA1
uniprotkb:Q6UQ80
uniprotkb:Q7Z2D8
uniprotkb:Q8IWW3
uniprotkb:Q8IWW4
uniprotkb:Q9UCZ4
uniprotkb:Q9UN57
uniprotkb:G8XRH6
uniprotkb:E7EWZ3
ensembl:ENSP00000328264.7
uniprotkb:B0FXJ1
uniprotkb:B8Q1L8
uniprotkb:B8Q1L9
uniprotkb:Q6UPP1
uniprotkb:Q86V80
Alternative identifier for interactor Bintact:EBI-5461341
uniprotkb:A2A2T1
uniprotkb:O95392
ensembl:ENSP00000372335.4
Aliases for Apsi-mi:oprm_human(display_long)
uniprotkb:OPRM1(gene name)
psi-mi:OPRM1(display_short)
uniprotkb:MOR1(gene name synonym)
uniprotkb:Mu opioid receptor(gene name synonym)
uniprotkb:Mu opiate receptor(gene name synonym)
Aliases for Bpsi-mi:nelfa_human(display_long)
uniprotkb:NELFA(gene name)
psi-mi:NELFA(display_short)
uniprotkb:WHSC2(gene name synonym)
uniprotkb:Wolf-Hirschhorn syndrome candidate 2 protein(gene name synonym)
uniprotkb:P/OKcl.15(orf name)
Interaction detection methodspsi-mi:"MI:0007"(anti tag coimmunoprecipitation)
First authorSokolina et al. (2017)
Identifier of the publicationpubmed:28298427
doi:10.15252/msb.20167430
imex:IM-26539
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0915"(physical association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-20796651
imex:IM-26539-25
Confidence scoreintact-miscore:0.51
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role ABait
Experimental role BPrey
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:F4A Figure EV4 Table EV6
comment:In the first approach, FLAG‐tagged GPCR interactors were overexpressed in mammalian cells, pulled‐down using anti‐FLAG antibody, subjected to SDS–PAGE, transferred to membranes, and probed with commercial antibody raised against their identified endogenous GPCR‐interaction partner.
full coverage:Only protein-protein interactions
curation depth:imex curation
NCBI Taxonomy identifier for the host organismtaxid:9606(human-293t)
taxid:9606(Homo sapiens HEK293T embryonic kidney cell)
Parameters of the interaction-
Creation date2018/07/06
Update date2018/10/16
negative Boolean valuefalse
Feature(s) for interactor Aflag tag:?-?
Feature(s) for interactor B-
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor AWestern blot
Participant identification method for interactor BWestern blot