Curation Details
Interaction ID: IM-26539-23

Unique identifier for interactor Auniprotkb:P16473
Unique identifier for interactor Buniprotkb:P21579
Alternative identifier for interactor Aintact:EBI-13939599
uniprotkb:A0PJU7
uniprotkb:F5GYU5
uniprotkb:G3V2A9
uniprotkb:Q16503
uniprotkb:Q8TB90
uniprotkb:Q96GT6
uniprotkb:Q9P1V4
uniprotkb:Q9ULA3
uniprotkb:Q9UPH3
Alternative identifier for interactor Bintact:EBI-524909
uniprotkb:Q6AI31
ensembl:ENSP00000261205.4
ensembl:ENSP00000376932.3
ensembl:ENSP00000447575.1
ensembl:ENSP00000515993.1
ensembl:ENSP00000515997.1
ensembl:ENSP00000515999.1
ensembl:ENSP00000516001.1
ensembl:ENSP00000516002.1
ensembl:ENSP00000516003.1
ensembl:ENSP00000516005.1
ensembl:ENSP00000516007.1
ensembl:ENSP00000516009.1
ensembl:ENSP00000516013.1
Aliases for Apsi-mi:tshr_human(display_long)
uniprotkb:TSHR(gene name)
psi-mi:TSHR(display_short)
uniprotkb:LGR3(gene name synonym)
uniprotkb:Thyroid-stimulating hormone receptor(gene name synonym)
Aliases for Bpsi-mi:syt1_human(display_long)
uniprotkb:Synaptotagmin I(gene name synonym)
uniprotkb:p65(gene name synonym)
uniprotkb:SYT1(gene name)
psi-mi:SYT1(display_short)
uniprotkb:SYT(gene name synonym)
uniprotkb:SVP65(gene name synonym)
Interaction detection methodspsi-mi:"MI:0007"(anti tag coimmunoprecipitation)
First authorSokolina et al. (2017)
Identifier of the publicationpubmed:28298427
doi:10.15252/msb.20167430
imex:IM-26539
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0915"(physical association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-20796644
imex:IM-26539-23
Confidence scoreintact-miscore:0.51
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role ABait
Experimental role BPrey
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:F4A Figure EV4 Table EV6
comment:In the first approach, FLAG‐tagged GPCR interactors were overexpressed in mammalian cells, pulled‐down using anti‐FLAG antibody, subjected to SDS–PAGE, transferred to membranes, and probed with commercial antibody raised against their identified endogenous GPCR‐interaction partner.
full coverage:Only protein-protein interactions
curation depth:imex curation
NCBI Taxonomy identifier for the host organismtaxid:9606(human-293t)
taxid:9606(Homo sapiens HEK293T embryonic kidney cell)
Parameters of the interaction-
Creation date2018/07/06
Update date2018/10/16
negative Boolean valuefalse
Feature(s) for interactor Aflag tag:?-?
Feature(s) for interactor B-
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor AWestern blot
Participant identification method for interactor BWestern blot