Curation Details
Interaction ID: IM-26539-1

Unique identifier for interactor Auniprotkb:O15354
Unique identifier for interactor Buniprotkb:P08195
Alternative identifier for interactor Aintact:EBI-15639515
ensembl:ENSP00000306449.2
uniprotkb:A4D0Y6
uniprotkb:O00348
uniprotkb:O14768
uniprotkb:Q8TD39
Alternative identifier for interactor Bintact:EBI-702356
ensembl:ENSP00000367122.2
intact:EBI-21352943
uniprotkb:J3KPF3
uniprotkb:Q13543
Aliases for Apsi-mi:gpr37_human(display_long)
uniprotkb:GPR37(gene name)
psi-mi:GPR37(display_short)
uniprotkb:Endothelin B receptor-like protein 1(gene name synonym)
uniprotkb:G-protein coupled receptor 37(gene name synonym)
uniprotkb:Parkin-associated endothelin receptor-like receptor(gene name synonym)
Aliases for Bpsi-mi:4f2_human(display_long)
uniprotkb:Lymphocyte activation antigen 4F2 large subunit(gene name synonym)
uniprotkb:4F2 heavy chain antigen(gene name synonym)
uniprotkb:SLC3A2(gene name)
psi-mi:SLC3A2(display_short)
uniprotkb:MDU1(gene name synonym)
uniprotkb:Solute carrier family 3 member 2(gene name synonym)
uniprotkb:4F2 cell-surface antigen heavy chain(gene name synonym)
Interaction detection methodspsi-mi:"MI:0007"(anti tag coimmunoprecipitation)
First authorSokolina et al. (2017)
Identifier of the publicationpubmed:28298427
doi:10.15252/msb.20167430
imex:IM-26539
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0915"(physical association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-20796600
imex:IM-26539-1
Confidence scoreintact-miscore:0.58
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role ABait
Experimental role BPrey
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:F4A Figure EV4 Table EV6
comment:In the first approach, FLAG‐tagged GPCR interactors were overexpressed in mammalian cells, pulled‐down using anti‐FLAG antibody, subjected to SDS–PAGE, transferred to membranes, and probed with commercial antibody raised against their identified endogenous GPCR‐interaction partner.
full coverage:Only protein-protein interactions
curation depth:imex curation
NCBI Taxonomy identifier for the host organismtaxid:9606(human-293t)
taxid:9606(Homo sapiens HEK293T embryonic kidney cell)
Parameters of the interaction-
Creation date2018/07/06
Update date2018/10/16
negative Boolean valuefalse
Feature(s) for interactor Aflag tag:?-?
Feature(s) for interactor B-
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor AWestern blot
Participant identification method for interactor BWestern blot