Curation Details
Interaction ID: IM-26522-5

Unique identifier for interactor Auniprotkb:P11309-2
Unique identifier for interactor Buniprotkb:P17252
Alternative identifier for interactor Aintact:EBI-1018633
intact:EBI-20775148
Alternative identifier for interactor Bintact:EBI-1383528
uniprotkb:Q96RE4
uniprotkb:Q15137
uniprotkb:Q32M72
uniprotkb:B5BU22
ensembl:ENSP00000408695.3
Aliases for Apsi-mi:p11309-2(display_long)
uniprotkb:Pim-1L(isoform synonym)
uniprotkb:PIM1(gene name)
psi-mi:PIM1(display_short)
Aliases for Bpsi-mi:kpca_human(display_long)
uniprotkb:PRKCA(gene name)
psi-mi:PRKCA(display_short)
uniprotkb:PKCA(gene name synonym)
uniprotkb:PRKACA(gene name synonym)
Interaction detection methodspsi-mi:"MI:0007"(anti tag coimmunoprecipitation)
First authorTakami et al. (2018)
Identifier of the publicationimex:IM-26522
pubmed:30017192
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0915"(physical association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-20765574
imex:IM-26522-5
Confidence scoreintact-miscore:0.54
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role ABait
Experimental role BPrey
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:f1d
comment:"\"Immunoprecipitation–western blotting revealed that transfection of pcDNA3.1-HA-PKCα-WT did not increase the phosphorylated PIM-1L-K158R. In contrast, transfection of pcDNA3.1-HA-PKCα-A25E increased the phosphorylated band of PIM-1L-K158R (Fig. 1D).\""
comment:"\"PKCα phosphorylated all of the PIM-1L mutants, but the phosphorylation levels of PIM-1L-K158R•S65A and -K158R•S65A•367A were lower than those of PIM-1L-K158R and -K158R•S367A (Fig. 1E).\""
curation depth:imex curation
full coverage:Only protein-protein interactions
NCBI Taxonomy identifier for the host organismtaxid:9606(human-293)
taxid:9606(Homo sapiens HEK293 embryonic kidney cell)
Parameters of the interaction-
Creation date2018/09/24
Update date2024/11/14
negative Boolean valuefalse
Feature(s) for interactor Aflag tag:?-?
mutation with no effect:158-158
Feature(s) for interactor Bmutation with no effect:25-25
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor AWestern blot
Participant identification method for interactor BWestern blot