Unique identifier for interactor A | uniprotkb:P11309-2 |
Unique identifier for interactor B | uniprotkb:P17252 |
Alternative identifier for interactor A | intact:EBI-1018633 intact:EBI-20775148 |
Alternative identifier for interactor B | intact:EBI-1383528 uniprotkb:Q96RE4 uniprotkb:Q15137 uniprotkb:Q32M72 uniprotkb:B5BU22 ensembl:ENSP00000408695.3 |
Aliases for A | psi-mi:p11309-2(display_long) uniprotkb:Pim-1L(isoform synonym) uniprotkb:PIM1(gene name) psi-mi:PIM1(display_short) |
Aliases for B | psi-mi:kpca_human(display_long) uniprotkb:PRKCA(gene name) psi-mi:PRKCA(display_short) uniprotkb:PKCA(gene name synonym) uniprotkb:PRKACA(gene name synonym) |
Interaction detection methods | psi-mi:"MI:0007"(anti tag coimmunoprecipitation) |
First author | Takami et al. (2018) |
Identifier of the publication | imex:IM-26522 pubmed:30017192 |
NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
Interaction types | psi-mi:"MI:0915"(physical association) |
Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
Interaction identifier(s) in the corresponding source database | intact:EBI-20765574 imex:IM-26522-5 |
Confidence score | intact-miscore:0.54 |
Complex expansion | - |
Biological role A | Unspecified role |
Biological role B | Unspecified role |
Experimental role A | Bait |
Experimental role B | Prey |
Interactor type A | Protein |
Interactor type B | Protein |
Annotations for the interaction | figure legend:f1d comment:"\"Immunoprecipitation–western blotting revealed that transfection of pcDNA3.1-HA-PKCα-WT did not increase the phosphorylated PIM-1L-K158R. In contrast, transfection of pcDNA3.1-HA-PKCα-A25E increased the phosphorylated band of PIM-1L-K158R (Fig. 1D).\"" comment:"\"PKCα phosphorylated all of the PIM-1L mutants, but the phosphorylation levels of PIM-1L-K158R•S65A and -K158R•S65A•367A were lower than those of PIM-1L-K158R and -K158R•S367A (Fig. 1E).\"" curation depth:imex curation full coverage:Only protein-protein interactions |
NCBI Taxonomy identifier for the host organism | taxid:9606(human-293) taxid:9606(Homo sapiens HEK293 embryonic kidney cell) |
Parameters of the interaction | - |
Creation date | 2018/09/24 |
Update date | 2024/11/14 |
negative Boolean value | false |
Feature(s) for interactor A | flag tag:?-? mutation with no effect:158-158 |
Feature(s) for interactor B | mutation with no effect:25-25 |
Stoichiometry for interactor A | - |
Stoichiometry for interactor B | - |
Participant identification method for interactor A | Western blot |
Participant identification method for interactor B | Western blot |