Unique identifier for interactor A | uniprotkb:P11309-2 |
Unique identifier for interactor B | uniprotkb:P17252 |
Alternative identifier for interactor A | intact:EBI-1018633 intact:EBI-20775148 |
Alternative identifier for interactor B | intact:EBI-1383528 uniprotkb:Q96RE4 uniprotkb:Q15137 uniprotkb:Q32M72 uniprotkb:B5BU22 ensembl:ENSP00000408695.3 |
Aliases for A | psi-mi:p11309-2(display_long) uniprotkb:Pim-1L(isoform synonym) uniprotkb:PIM1(gene name) psi-mi:PIM1(display_short) |
Aliases for B | psi-mi:kpca_human(display_long) uniprotkb:PRKCA(gene name) psi-mi:PRKCA(display_short) uniprotkb:PKCA(gene name synonym) uniprotkb:PRKACA(gene name synonym) |
Interaction detection methods | psi-mi:"MI:0424"(protein kinase assay) |
First author | Takami et al. (2018) |
Identifier of the publication | imex:IM-26522 pubmed:30017192 |
NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
Interaction types | psi-mi:"MI:0217"(phosphorylation reaction) |
Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
Interaction identifier(s) in the corresponding source database | intact:EBI-20765529 imex:IM-26522-1 |
Confidence score | intact-miscore:0.54 |
Complex expansion | - |
Biological role A | Enzyme target |
Biological role B | Enzyme |
Experimental role A | Neutral component |
Experimental role B | Neutral component |
Interactor type A | Protein |
Interactor type B | Protein |
Annotations for the interaction | figure legend:f1c f1e comment:"\"The kinase-dead PIM-1L-K158R [29] was used to avoid PIM-1L autophosphorylation. No phosphorylation bands were observed when PIM-1L-K158R alone was incubated (Fig. 1C). PIM-1L-K158R phosphorylation bands (44 kDa) were detected when incubated with PKA and PKCα (Fig. 1C).\"" comment:"\"PKCα phosphorylated all of the PIM-1L mutants, but the phosphorylation levels of PIM-1L-K158R•S65A and -K158R•S65A•367A were lower than those of PIM-1L-K158R and -K158R•S367A (Fig. 1E).\"" curation depth:imex curation full coverage:Only protein-protein interactions |
NCBI Taxonomy identifier for the host organism | taxid:-1(in vitro) taxid:-1(In vitro) |
Parameters of the interaction | - |
Creation date | 2018/09/24 |
Update date | 2024/11/14 |
negative Boolean value | false |
Feature(s) for interactor A | flag tag:?-? mutation with no effect:158-158 mutation decreasing interaction rate:65-65 phosphorylated residue:65-65 |
Feature(s) for interactor B | - |
Stoichiometry for interactor A | - |
Stoichiometry for interactor B | - |
Participant identification method for interactor A | Autoradiography |
Participant identification method for interactor B | Autoradiography |