Curation Details
Interaction ID: IM-24904-9

Unique identifier for interactor Auniprotkb:Q5VY09
Unique identifier for interactor Buniprotkb:P67775
Alternative identifier for interactor Aintact:EBI-1774000
uniprotkb:B2RBV3
ensembl:ENSP00000356549.4
uniprotkb:Q8WY68
uniprotkb:Q9NY49
uniprotkb:Q9NZP9
Alternative identifier for interactor Bintact:EBI-712311
ensembl:ENSP00000418447.1
uniprotkb:P05323
uniprotkb:P13197
Aliases for Apsi-mi:ier5_human(display_long)
uniprotkb:IER5(gene name)
psi-mi:IER5(display_short)
uniprotkb:PP4583(orf name)
uniprotkb:SBBI48(orf name)
Aliases for Bpsi-mi:pp2aa_human(display_long)
uniprotkb:PPP2CA(gene name)
psi-mi:PPP2CA(display_short)
uniprotkb:Replication protein C(gene name synonym)
Interaction detection methodspsi-mi:"MI:0096"(pull down)
First authorKawabata et al. (2015)
Identifier of the publicationpubmed:26496226
imex:IM-24904
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0915"(physical association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-11475274
imex:IM-24904-9
Confidence scoreintact-miscore:0.40
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role ABait
Experimental role BPrey
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:f3d
comment:"\"The GST- 49C fusion failed to retain B55b-HA, suggesting that the N-terminal IER2 homology region is necessary for the binding. The GST-N246 fusion lacking the C-terminal 81 amino acids exhibited reduced binding ability relative to GST-FL; however, the C-terminal region itself failed to retain B55b-HA (GST-234C). The GST-ID fusion lacking the internal region of IER5 efficiently bound to B55b-HA. Therefore, binding to B55b is mediated by both the N-terminal and C-terminal regions of IER5, where the C-terminal region enhances the binding only in the presence of the N-terminal region\""
full coverage:Only protein-protein interactions
curation depth:imex curation
NCBI Taxonomy identifier for the host organismtaxid:9606(human-hela)
taxid:9606(Homo sapiens HeLa epitheloid cervical carcinoma cell)
Parameters of the interaction-
Creation date2016/01/07
Update date2016/01/07
negative Boolean valuefalse
Feature(s) for interactor Aglutathione s tranferase tag:?-?
Feature(s) for interactor B-
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor AAnti tag western blot
Participant identification method for interactor BWestern blot