| Unique identifier for interactor A | uniprotkb:Q13148 |
| Unique identifier for interactor B | uniprotkb:Q13148 |
| Alternative identifier for interactor A | intact:EBI-372899 intact:EBI-2821405 uniprotkb:A4GUK4 uniprotkb:A4GUK5 uniprotkb:A4GUK6 uniprotkb:B2R629 uniprotkb:Q53H27 ensembl:ENSP00000240185.4 ensembl:ENSP00000491203.1 uniprotkb:B4DJ45 uniprotkb:Q6FI92 uniprotkb:E2PU12 uniprotkb:Q96DJ0 |
| Alternative identifier for interactor B | intact:EBI-372899 intact:EBI-2821405 uniprotkb:A4GUK4 uniprotkb:A4GUK5 uniprotkb:A4GUK6 uniprotkb:B2R629 uniprotkb:Q53H27 ensembl:ENSP00000240185.4 ensembl:ENSP00000491203.1 uniprotkb:B4DJ45 uniprotkb:Q6FI92 uniprotkb:E2PU12 uniprotkb:Q96DJ0 |
| Aliases for A | psi-mi:tadbp_human(display_long) uniprotkb:TARDBP(gene name) psi-mi:TARDBP(display_short) uniprotkb:TDP43(gene name synonym) |
| Aliases for B | psi-mi:tadbp_human(display_long) uniprotkb:TARDBP(gene name) psi-mi:TARDBP(display_short) uniprotkb:TDP43(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0007"(anti tag coimmunoprecipitation) |
| First author | Che et al. (2015) |
| Identifier of the publication | imex:IM-24545 pubmed:26099433 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0915"(physical association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-11292973 imex:IM-24545-3 |
| Confidence score | intact-miscore:0.97 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Prey |
| Experimental role B | Bait |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f1b f1c f1d f2b comment:"\"We also tested whether TDP-35 associates with endogenous TDP-43. The data exhibited that FLAG-TDP-35 could immunoprecipitate the endogenous TDP-43. The restoration experiments by adding ssDNA in the RNase-A treated sample suggested that single-strand DNA could improve the association of TDP-35 with TDP-43 (Fig. 1c, lane 5), but it failed to act on TDP-25A with TDP-43 (Fig. 1c, lane 11). To test whether DNA was involved in the association, we conducted immunoprecipitation on the cell lysates treated with deoxyribonuclease (DNase). Compared with RNase-A treatment, DNase treatment did not affect sequestration of TDP-43 by TDP-35 inclusions (Fig. 1d, lane 8). Altogether, these results demonstrate that sequestration of full-length TDP-43 by the cytoplasmic TDP-35 inclusions is mediated by RNA.\"" full coverage:Only protein-protein interactions curation depth:imex curation |
| NCBI Taxonomy identifier for the host organism | taxid:9606(human-293t) taxid:9606(Homo sapiens HEK293T embryonic kidney cell) |
| Parameters of the interaction | - |
| Creation date | 2015/09/18 |
| Update date | 2015/09/30 |
| negative Boolean value | false |
| Feature(s) for interactor A | - |
| Feature(s) for interactor B | mutation decreasing interaction:147-147,149-149 flag tag:?-? binding-associated region:90-414 mutation disrupting interaction:229-229,147-147,149-149,231-231 |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Anti tag western blot |
| Participant identification method for interactor B | Anti tag western blot |