Curation Details
Interaction ID: IM-24529-1

Unique identifier for interactor Auniprotkb:Q96SW2
Unique identifier for interactor Buniprotkb:Q13422
Alternative identifier for interactor Aintact:EBI-2510250
uniprotkb:B2R6H4
uniprotkb:C9IZA9
uniprotkb:C9JAH6
uniprotkb:Q6AI62
uniprotkb:Q9UHW4
uniprotkb:Q6NVZ0
ensembl:ENSP00000231948.4
Alternative identifier for interactor Bintact:EBI-745305
uniprotkb:O00598
uniprotkb:Q8WVA3
uniprotkb:Q53XL2
uniprotkb:B4E0Z1
uniprotkb:A4D260
uniprotkb:D3DVM5
uniprotkb:Q69BM4
intact:EBI-28969803
ensembl:ENSP00000331614.3
Aliases for Apsi-mi:crbn_human(display_long)
uniprotkb:CRBN(gene name)
psi-mi:CRBN(display_short)
uniprotkb:AD-006(orf name)
Aliases for Bpsi-mi:ikzf1_human(display_long)
uniprotkb:Ikaros family zinc finger protein 1(gene name synonym)
uniprotkb:Lymphoid transcription factor LyF-1(gene name synonym)
uniprotkb:IKZF1(gene name)
psi-mi:IKZF1(display_short)
uniprotkb:IK1(gene name synonym)
uniprotkb:IKAROS(gene name synonym)
uniprotkb:LYF1(gene name synonym)
uniprotkb:ZNFN1A1(gene name synonym)
Interaction detection methodspsi-mi:"MI:0096"(pull down)
First authorLiu et al. (2015)
Identifier of the publicationimex:IM-24529
pubmed:26183205
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0915"(physical association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-11189384
imex:IM-24529-1
Confidence scoreintact-miscore:0.40
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role ABait
Experimental role BPrey
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:f3a
comment:"\"We coexpressed FLAG-IKZF1 with Strep-CRBN in HEK293T cells and treated the cells with lenalidomide, DRB, and Rosc along with a proteasome inhibitor MG132. We then used Strep-Tactin agarose beads to affinity-purify Strep-CRBN and its interacting proteins and blotted the purified samples with anti-FLAG and anti-CRBN antibodies. Immunoblotting results showed that IKZF1 was readily detected in the pulldown when the cells were treated with lenalidomide (Fig. 3A).\""
curation depth:imex curation
full coverage:Only protein-protein interactions
NCBI Taxonomy identifier for the host organismtaxid:-1(in vitro)
taxid:-1(In vitro)
Parameters of the interaction-
Creation date2015/09/23
Update date2024/11/14
negative Boolean valuefalse
Feature(s) for interactor Astrep tag:?-?
Feature(s) for interactor Bflag tag:?-?
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor AWestern blot
Participant identification method for interactor BWestern blot