Curation Details
Interaction ID: IM-23858-2

Unique identifier for interactor Auniprotkb:Q00613
Unique identifier for interactor Buniprotkb:P02545
Alternative identifier for interactor Aintact:EBI-719620
ensembl:ENSP00000431512.1
ensembl:ENSP00000493830.1
uniprotkb:A8MW26
uniprotkb:Q53XT4
uniprotkb:A8K4L0
Alternative identifier for interactor Bintact:EBI-351935
uniprotkb:Q5TCJ3
ensembl:ENSP00000355292.6
ensembl:ENSP00000357283.4
ensembl:ENSP00000502256.1
ensembl:ENSP00000506771.1
uniprotkb:D3DVB0
intact:EBI-28988787
uniprotkb:B4DI32
uniprotkb:E7EUI9
uniprotkb:P02546
uniprotkb:Q969I8
uniprotkb:Q96JA2
uniprotkb:D6RAQ3
uniprotkb:Q5I6Y4
uniprotkb:Q5I6Y6
uniprotkb:Q6UYC3
uniprotkb:Q5TCJ2
Aliases for Apsi-mi:hsf1_human(display_long)
uniprotkb:Heat shock transcription factor 1(gene name synonym)
uniprotkb:HSF1(gene name)
psi-mi:HSF1(display_short)
uniprotkb:HSTF1(gene name synonym)
Aliases for Bpsi-mi:lmna_human(display_long)
uniprotkb:LMNA(gene name)
psi-mi:LMNA(display_short)
uniprotkb:LMN1(gene name synonym)
Interaction detection methodspsi-mi:"MI:0029"(cosedimentation through density gradient)
First authorDai et al. (2015)
Identifier of the publicationimex:IM-23858
pubmed:25425574
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0403"(colocalization)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-10682923
imex:IM-23858-2
Confidence scoreintact-miscore:0.35
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role ANeutral component
Experimental role BNeutral component
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:f3i f3j
comment:"\"Despite no impacts on HSF1 expression, AMPKaCA impaired constitutive nuclear translocation of HSF1WT in HEK293T cells, indicated by diminished nuclear–cytoplasmic ratio of HSF1 levels (Fig 3I). S121A mutation not only enhanced HSF1 nuclear translocation under basal conditions but also rendered HSF1 resistant to the effect of AMPKaCA (Fig 3I). Similarly, while metformin impaired HSF1WT nuclear translocation, it exerted little effect on HSF1S121A mutants (Fig 3J). Thus, this defect in nuclear translocation due to Ser121 phosphorylation at least in part contributes to reduced HSF1–DNA binding\""
full coverage:Only protein-protein interactions
curation depth:imex curation
NCBI Taxonomy identifier for the host organismtaxid:9606(human-293t)
taxid:9606(Homo sapiens HEK293T embryonic kidney cell)
Parameters of the interaction-
Creation date2015/02/18
Update date2015/02/20
negative Boolean valuefalse
Feature(s) for interactor Aflag tag:?-?
Feature(s) for interactor B-
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor AAnti tag western blot
Participant identification method for interactor BWestern blot