Curation Details
Interaction ID: IM-23790-2

Unique identifier for interactor Auniprotkb:Q96QK1
Unique identifier for interactor Buniprotkb:Q7Z3C6
Alternative identifier for interactor Aintact:EBI-1054634
ensembl:ENSP00000299138.7
uniprotkb:Q561W2
uniprotkb:Q9H016
uniprotkb:Q9H096
uniprotkb:Q9H4P3
uniprotkb:Q9H8J0
uniprotkb:Q9NRS7
uniprotkb:Q9NVG2
uniprotkb:Q9NX80
uniprotkb:Q9NZK2
Alternative identifier for interactor Bintact:EBI-727146
ensembl:ENSP00000355173.4
ensembl:ENSP00000379983.2
ensembl:ENSP00000386710.1
uniprotkb:Q6P0N7
uniprotkb:Q7Z317
uniprotkb:Q7Z320
uniprotkb:Q8NDK6
uniprotkb:Q8WU65
uniprotkb:Q9BVL5
uniprotkb:Q9H6L1
uniprotkb:Q9HAG7
uniprotkb:Q3ZAQ6
Aliases for Apsi-mi:vps35_human(display_long)
uniprotkb:Vesicle protein sorting 35(gene name synonym)
uniprotkb:Maternal-embryonic 3(gene name synonym)
uniprotkb:VPS35(gene name)
psi-mi:VPS35(display_short)
uniprotkb:MEM3(gene name synonym)
uniprotkb:TCCCTA00141(orf name)
Aliases for Bpsi-mi:atg9a_human(display_long)
uniprotkb:APG9-like 1(gene name synonym)
uniprotkb:ATG9A(gene name)
psi-mi:ATG9A(display_short)
uniprotkb:APG9L1(gene name synonym)
uniprotkb:mATG9(gene name synonym)
Interaction detection methodspsi-mi:"MI:0416"(fluorescence microscopy)
First authorPopovic et al. (2014)
Identifier of the publicationpubmed:24603492
imex:IM-23790
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0403"(colocalization)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-10104110
imex:IM-23790-2
Confidence scoreintact-miscore:0.46
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role ANeutral component
Experimental role BNeutral component
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:f1a f1c sf1a sf1c
comment:"\"In U2OS cells stably expressing mCherry-ATG9, endogenous TBC1D5, retromer subunit VPS35, and mCherry-ATG9 co-localized in steady state as well as during autophagy induced by the mTOR inhibitor KU0063974 (Fig1A). Closer inspection of ATG9-containing vesicles showed that ATG9 does not enter retromer-associated endosomal tubules (Supplementary Fig S1A). Co-localization of TBC1D5 with ATG9 was steadily high in both tested conditions (Fig 1B), while retromer co-localization decreased upon autophagy induction (Fig 1C). Endogenous ATG9, LC3, and TBC1D5 also co-localized in cells (Supplementary Fig S1B).\""
full coverage:Only protein-protein interactions
curation depth:imex curation
NCBI Taxonomy identifier for the host organismtaxid:9606(human-u2os)
taxid:9606(Homo sapiens U2OS osteosarcoma cell)
Parameters of the interaction-
Creation date2014/10/06
Update date2015/02/04
negative Boolean valuefalse
Feature(s) for interactor A-
Feature(s) for interactor Bmcherry fluorescent protein tag:?-?
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor AImmunostaining
Participant identification method for interactor BTag visualisation by fluorescence