| Unique identifier for interactor A | uniprotkb:P11413 |
| Unique identifier for interactor B | uniprotkb:P11413 |
| Alternative identifier for interactor A | intact:EBI-4289891 ensembl:ENSP00000377192.3 ensembl:ENSP00000377194.2 ensembl:ENSP00000512624.1 ensembl:ENSP00000512625.1 uniprotkb:Q8IU88 uniprotkb:D3DWX9 uniprotkb:Q16000 uniprotkb:Q16765 uniprotkb:Q8IUA6 uniprotkb:Q8IU70 uniprotkb:Q96PQ2 |
| Alternative identifier for interactor B | intact:EBI-4289891 ensembl:ENSP00000377192.3 ensembl:ENSP00000377194.2 ensembl:ENSP00000512624.1 ensembl:ENSP00000512625.1 uniprotkb:Q8IU88 uniprotkb:D3DWX9 uniprotkb:Q16000 uniprotkb:Q16765 uniprotkb:Q8IUA6 uniprotkb:Q8IU70 uniprotkb:Q96PQ2 |
| Aliases for A | psi-mi:g6pd_human(display_long) uniprotkb:G6PD(gene name) psi-mi:G6PD(display_short) |
| Aliases for B | psi-mi:g6pd_human(display_long) uniprotkb:G6PD(gene name) psi-mi:G6PD(display_short) |
| Interaction detection methods | psi-mi:"MI:0007"(anti tag coimmunoprecipitation) |
| First author | Wang et al. (2014) |
| Identifier of the publication | imex:IM-23514 pubmed:24769394 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0915"(physical association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-9830927 imex:IM-23514-1 |
| Confidence score | intact-miscore:0.57 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Bait |
| Experimental role B | Prey |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:F2B F2D SF5 F4E comment:"\"To test this hypothesis, we determined the interaction between two differentially tagged G6PD proteins, G6PD-Flag and GFP-G6PD, in HEK293T cells. We found that mutation of K171 to either R (K171R) or Q (K171Q) did not affect the interaction between G6PD subunits (Fig 2B). In contrast, substitution of K386Q, but not K386R, impaired the interaction between G6PD subunits (Fig 2B). Strikingly, substitution of K403Q, but not K403R, entirely disrupted the interaction between G6PD subunits (Fig 2B). In addition, glutaraldehyde cross-linking assay demonstrated that the K403Q mutant displayed impaired ability to form dimers when compared to wildtype G6PD or the K403R mutant (Fig 2C). Moreover, NAM treatment decreased the binding by approximately 55% between the two differentially tagged proteins of wild-type G6PD in a dose-dependent manner (Fig 2D and Supplementary Fig S5). NAM treatment, however, failed to affect the interaction between Flag-tagged and GFP-tagged K403R mutant of G6PD (Fig 2D), further suggesting that K403 acetylation largely hinders the interaction between G6PD subunits.\"" comment:"\"Co-expression of SIRT2, but not the catalytic inactive SIRT2H187Y mutant, increased the interaction between two differentially tagged G6PD, and this effect could be diminished by NAM treatment (Fig 4E).\"" full coverage:Only protein-protein interactions curation depth:imex curation |
| NCBI Taxonomy identifier for the host organism | taxid:9606(human-293t) taxid:9606(Homo sapiens HEK293T embryonic kidney cell) |
| Parameters of the interaction | - |
| Creation date | 2014/10/07 |
| Update date | 2014/10/16 |
| negative Boolean value | false |
| Feature(s) for interactor A | flag tag:?-? mutation disrupting interaction:403-403 |
| Feature(s) for interactor B | green fluorescent protein tag:?-? |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Anti tag western blot |
| Participant identification method for interactor B | Anti tag western blot |