Curation Details
Interaction ID: IM-23185-1

Unique identifier for interactor Auniprotkb:P63000
Unique identifier for interactor Buniprotkb:P02699
Alternative identifier for interactor Aintact:EBI-413628
ensembl:ENSP00000258737.7
uniprotkb:O95501
uniprotkb:P15154
uniprotkb:Q3Y4D3
uniprotkb:Q5JAA8
uniprotkb:Q9BTB4
Alternative identifier for interactor Bintact:EBI-8592832
intact:MINT-1507462
ensembl:ENSBTAP00000001730.5
Aliases for Apsi-mi:rac1_human(display_long)
uniprotkb:RAC1(gene name)
psi-mi:RAC1(display_short)
uniprotkb:MIG5(orf name)
uniprotkb:p21-Rac1(gene name synonym)
uniprotkb:Ras-like protein TC25(gene name synonym)
uniprotkb:Cell migration-inducing gene 5 protein(gene name synonym)
uniprotkb:TC25(gene name synonym)
Aliases for Bpsi-mi:opsd_bovin(display_long)
uniprotkb:RHO(gene name)
psi-mi:RHO(display_short)
Interaction detection methodspsi-mi:"MI:0107"(surface plasmon resonance)
First authorKöster et al. (2014)
Identifier of the publicationpubmed:25243418
imex:IM-23185
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9913(bovin)
taxid:9913("Bos taurus (Bovine)")
Interaction typespsi-mi:"MI:0407"(direct interaction)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-9822518
imex:IM-23185-1
Confidence scoreintact-miscore:0.44
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role APrey
Experimental role BBait
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:f3
comment:"\"Rac1 bound to immobilized rhodopsin in a concentration dependent manner (Figure 3A).\""
comment:"\"The binding process was not triggered by light, since Rac1 bound to dark-adapted rhodopsin in a similar manner as to light-activated rhodopsin and binding of Rac1 to rhodopsin was not reversed by adding GTPγS (Figure 3B), which can be used to rapidly dissociate Gt from rhodopsin that is immobilized on a sensor chip surface\""
comment:Further, the amount of Rac1 interacting with rhodopsin was not increased by illumination as can be seen in Figure 3C, where rhodopsin was bleached after Rac1 was injected into the flow cell and the sensorgram was compared with a recording done in darkness
full coverage:Only protein-protein interactions
curation depth:imex curation
NCBI Taxonomy identifier for the host organismtaxid:-1(in vitro)
taxid:-1(In vitro)
Parameters of the interaction-
Creation date2014/09/22
Update date2014/10/16
negative Boolean valuefalse
Feature(s) for interactor A-
Feature(s) for interactor B-
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor APredetermined participant
Participant identification method for interactor BPredetermined participant