| Unique identifier for interactor A | uniprotkb:P16333 |
| Unique identifier for interactor B | uniprotkb:Q13191 |
| Alternative identifier for interactor A | intact:EBI-389883 uniprotkb:B7Z751 intact:EBI-3448658 uniprotkb:D3DNE3 ensembl:ENSP00000288986.2 ensembl:ENSP00000417273.1 |
| Alternative identifier for interactor B | intact:EBI-744027 uniprotkb:Q63Z43 uniprotkb:A8K9S7 uniprotkb:Q13192 uniprotkb:Q13193 uniprotkb:Q3LIC0 uniprotkb:Q8IVC5 uniprotkb:B7WNM4 intact:EBI-28969458 ensembl:ENSP00000377598.4 intact:EBI-8051403 intact:MINT-7907019 |
| Aliases for A | psi-mi:nck1_human(display_long) uniprotkb:NCK1(gene name) psi-mi:NCK1(display_short) uniprotkb:NCK(gene name synonym) uniprotkb:Cytoplasmic protein NCK1(gene name synonym) uniprotkb:NCK adapter protein 1(gene name synonym) uniprotkb:SH2/SH3 adapter protein NCK-alpha(gene name synonym) |
| Aliases for B | psi-mi:cblb_human(display_long) uniprotkb:Signal transduction protein CBL-B(gene name synonym) uniprotkb:SH3-binding protein CBL-B(gene name synonym) uniprotkb:Casitas B-lineage lymphoma proto-oncogene b(gene name synonym) uniprotkb:RING finger protein 56(gene name synonym) uniprotkb:CBLB(gene name) psi-mi:CBLB(display_short) uniprotkb:RNF56(gene name synonym) uniprotkb:Nbla00127(orf name) uniprotkb:RING-type E3 ubiquitin transferase CBL-B(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0007"(anti tag coimmunoprecipitation) |
| First author | Joseph et al. (2014) |
| Identifier of the publication | imex:IM-23171 pubmed:25218436 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0914"(association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-9694846 imex:IM-23171-4 |
| Confidence score | intact-miscore:0.35 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Bait |
| Experimental role B | Prey |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f6b f6c f6d comment:"\"293T cells were co-transfected with YFP-tagged Nck constructs encoding Nck-wt, or each of the SH3 mutant molecules, together with HA-Ub and Cbl-b. As shown in Figure 6B, each of the Nck-SH3-mutants exhibited a substantial decrease in their interaction with Cbl-b, and in Nck ubiquitylation. Moreover, a triple Nck-SH3 mutant (3SH3*) did not interact with Cbl-b at all, and was substantially less ubiquitylated (Fig. 6C).\"" comment:"\"To confirm this observation in T cells, Jurkat T-cell lines stably expressing the YFPNck constructs were prepared and analyzed (Fig. 6D). As in 293T cells, the molecular association of Cbl-b with each individual Nck-SH3-mutant was abolished. Furthermore, Nck ubiquitylation was correspondingly decreased. Thus, the three SH3 domains of Nck are all required for stable interaction with Cbl-b, and consequently, for Nck ubiquitylation.\"" curation depth:imex curation full coverage:Only protein-protein interactions |
| NCBI Taxonomy identifier for the host organism | taxid:9606(human) taxid:9606(Homo sapiens) |
| Parameters of the interaction | - |
| Creation date | 2014/08/28 |
| Update date | 2024/11/30 |
| negative Boolean value | false |
| Feature(s) for interactor A | yellow fluorescent protein tag:?-? mutation disrupting interaction:229-229,38-38,143-143 |
| Feature(s) for interactor B | - |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Western blot |
| Participant identification method for interactor B | Western blot |