| Unique identifier for interactor A | uniprotkb:Q9BPX6 |
| Unique identifier for interactor B | uniprotkb:Q9BPX6 |
| Alternative identifier for interactor A | intact:EBI-2371996 uniprotkb:O75785 uniprotkb:Q9H9N6 uniprotkb:Q9UFX0 uniprotkb:A8MV96 uniprotkb:B3KN20 uniprotkb:B4DJH9 uniprotkb:B4DPI1 uniprotkb:B5MBY3 uniprotkb:D3YTJ3 uniprotkb:A0A0U1RRK1 ensembl:ENSP00000354415.5 |
| Alternative identifier for interactor B | intact:EBI-2371996 uniprotkb:O75785 uniprotkb:Q9H9N6 uniprotkb:Q9UFX0 uniprotkb:A8MV96 uniprotkb:B3KN20 uniprotkb:B4DJH9 uniprotkb:B4DPI1 uniprotkb:B5MBY3 uniprotkb:D3YTJ3 uniprotkb:A0A0U1RRK1 ensembl:ENSP00000354415.5 |
| Aliases for A | psi-mi:micu1_human(display_long) uniprotkb:MICU1(gene name) psi-mi:MICU1(display_short) uniprotkb:Calcium-binding atopy-related autoantigen 1(gene name synonym) uniprotkb:CBARA1(gene name synonym) uniprotkb:CALC(gene name synonym) uniprotkb:Atopy-related autoantigen CALC(gene name synonym) |
| Aliases for B | psi-mi:micu1_human(display_long) uniprotkb:MICU1(gene name) psi-mi:MICU1(display_short) uniprotkb:Calcium-binding atopy-related autoantigen 1(gene name synonym) uniprotkb:CBARA1(gene name synonym) uniprotkb:CALC(gene name synonym) uniprotkb:Atopy-related autoantigen CALC(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0028"(cosedimentation in solution) |
| First author | Wang et al. (2014) |
| Identifier of the publication | imex:IM-23081 pubmed:24514027 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0407"(direct interaction) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-9550961 imex:IM-23081-4 |
| Confidence score | intact-miscore:0.56 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Neutral component |
| Experimental role B | Neutral component |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:sf3c f2e f2f f3f f3g comment:"\"To verify this hypothesis, we determined the oligomerization state of MICU1-xtal-deltaC. This mutant exhibited a predominant peak corresponding to the MICU1 dimer both in the absence and in the presence of Ca2+ (Fig 2E, F and Supplementary Fig 3C), suggesting that the C-helix is necessary for the formation of the Ca2+-free MICU1-xtal hexamer and substantially contributed to the formation of multiple oligomers of Ca2+-bound MICU1-xtal.\"" comment:"\"Within the Ca2+-free MICU1-xtal dimer interface, the two monomers primarily interact through a salt bridge. Asp376 from one monomer interacts with Arg221 from the reciprocal monomer via a salt bridge (Fig 3D). The R221A or D376A single mutation or the R221AD376A double mutation of MICU1-xtal-deltaC converted the dimer into the monomer in the absence of Ca2+ (Fig 3F). The Ca2+-bound MICU1-xtal-deltaC dimer interface is formed by hydrogen bonding and hydrophobic interactions (Fig 3E)\"" comment:"\"The R221A single mutation of MICU1- xtal-deltaC was unable to disrupt the dimer formation in the presence of Ca2+. The F383A-H385A double mutation of MICU1-xtal-deltaC converted the dimer into the monomer, and the dimer mixture had an approximate ratio of 1:1 in the presence of Ca2+ (Fig 3G).\"" curation depth:imex curation full coverage:Only protein-protein interactions |
| NCBI Taxonomy identifier for the host organism | taxid:-1(in vitro) taxid:-1(In vitro) |
| Parameters of the interaction | - |
| Creation date | 2014/06/17 |
| Update date | 2024/11/28 |
| negative Boolean value | false |
| Feature(s) for interactor A | mutation disrupting interaction:221-221 binding-associated region:97-444 |
| Feature(s) for interactor B | mutation disrupting interaction:221-221 binding-associated region:97-444 |
| Stoichiometry for interactor A | 2 |
| Stoichiometry for interactor B | 0 |
| Participant identification method for interactor A | Predetermined participant |
| Participant identification method for interactor B | Predetermined participant |