| Unique identifier for interactor A | uniprotkb:O14519 |
| Unique identifier for interactor B | uniprotkb:O95983 |
| Alternative identifier for interactor A | intact:EBI-1052532 ensembl:ENSP00000261692.2 intact:EBI-10988349 uniprotkb:F5GYA4 |
| Alternative identifier for interactor B | intact:EBI-1783068 uniprotkb:A8K4B7 uniprotkb:D6W5Z2 ensembl:ENSP00000412302.2 uniprotkb:Q6PIL9 uniprotkb:Q6PJZ9 uniprotkb:Q86XF4 |
| Aliases for A | psi-mi:cdka1_human(display_long) uniprotkb:Putative oral cancer suppressor(gene name synonym) uniprotkb:Deleted in oral cancer 1(gene name synonym) uniprotkb:CDK2AP1(gene name) psi-mi:CDK2AP1(display_short) uniprotkb:CDKAP1(gene name synonym) uniprotkb:DOC1(gene name synonym) |
| Aliases for B | psi-mi:mbd3_human(display_long) uniprotkb:MBD3(gene name) psi-mi:MBD3(display_short) uniprotkb:Methyl-CpG-binding protein MBD3(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0096"(pull down) |
| First author | Kloet et al. (2014) |
| Identifier of the publication | imex:IM-23066 pubmed:25123934 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0914"(association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-9692524 imex:IM-23066-2 |
| Confidence score | intact-miscore:0.53 |
| Complex expansion | psi-mi:"MI:1060"(spoke expansion) |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Bait |
| Experimental role B | Prey |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f2 comment:"\"To further study the potential dynamics of NuRD subunit interactions, a SILAC-based subunit exchange assay was used (Fig 2A) [15]. HeLa cells expressing GFP-CDK2AP-1 were labeled in culture with heavy amino acids (Forward) or light amino acids (Reverse). Similarly, WT HeLa cells were also labeled with light or heavy amino acids. GFP pulldowns were performed immediately after mixing the nuclear extracts (T0) or after overnight incubation (TON). Proteins that are more dynamically associated with NuRD will dissociate from the complex during the overnight incubation step and may be replaced by proteins from the other, differentially labeled extract. This eventually results in a decrease of detected SILAC ratios, where dynamic core subunits and/or interactors will move towards the background cloud in the scatter plot.At T0, all NuRD core subunits are significantly enriched according to boxplot statistics (Fig 2B). However, after overnight incubation, RBBP4 and -7 clearly separate from the other NuRD subunits and migrate towards the background cloud (Fig 2C). To more directly compare the 2 scatter plots, the difference in forward and reverse ratios between the plots was visualized in a graph (Fig 2D). A protein with no change in ratios between experiments would have a value of 0. This graph clearly shows that RBBP4 and -7 are the most dynamic NuRD core subunits. These observations are in agreement with recent structural studies, which suggest that MTA and histone H4 compete for RBBP binding [20]. Furthermore, RBBP4 and -7 are part of many different protein complexes other than NuRD (Sin3 complex, PRC2), which also could explain their observed dynamic behavior [21, 22].\"" full coverage:Only protein-protein interactions curation depth:imex curation |
| NCBI Taxonomy identifier for the host organism | taxid:9606(human-hela) taxid:9606(Homo sapiens HeLa epitheloid cervical carcinoma cell) |
| Parameters of the interaction | - |
| Creation date | 2014/08/20 |
| Update date | 2014/10/16 |
| negative Boolean value | false |
| Feature(s) for interactor A | green fluorescent protein tag:?-? |
| Feature(s) for interactor B | - |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Identification by mass spectrometry |
| Participant identification method for interactor B | Identification by mass spectrometry |