| Unique identifier for interactor A | uniprotkb:O95983 |
| Unique identifier for interactor B | uniprotkb:Q96KM6 |
| Alternative identifier for interactor A | intact:EBI-1783068 uniprotkb:A8K4B7 uniprotkb:D6W5Z2 ensembl:ENSP00000412302.2 uniprotkb:Q6PIL9 uniprotkb:Q6PJZ9 uniprotkb:Q86XF4 |
| Alternative identifier for interactor B | intact:EBI-1049952 ensembl:ENSP00000358904.1 uniprotkb:Q08AK9 uniprotkb:Q9ULM4 |
| Aliases for A | psi-mi:mbd3_human(display_long) uniprotkb:MBD3(gene name) psi-mi:MBD3(display_short) uniprotkb:Methyl-CpG-binding protein MBD3(gene name synonym) |
| Aliases for B | psi-mi:z512b_human(display_long) uniprotkb:ZNF512B(gene name) psi-mi:ZNF512B(display_short) uniprotkb:KIAA1196(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0096"(pull down) |
| First author | Kloet et al. (2014) |
| Identifier of the publication | imex:IM-23066 pubmed:25123934 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0914"(association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-9691985 imex:IM-23066-1 |
| Confidence score | intact-miscore:0.35 |
| Complex expansion | psi-mi:"MI:1060"(spoke expansion) |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Bait |
| Experimental role B | Prey |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f1 comment:"\"To determine the stability of the NuRD complex, several NP-40 and NaCl concentrations were used during the affinity purifications (Fig 1B-D). In each of these pull-downs, known NuRD core subunits were identified. In addition, several previously described NuRD interactors were also detected, including ZMYND8, ZNF592, and SALL4 [8, 14, 17]. Interestingly, we did not pulldown LSD1 in any of our MBD3-GFP purifications. LSD1 has previously been reported as a putative subunit of the NuRD complex [18] but our data on MBD3/NuRD does not agree with this observation. Next, the intensity-based absolute quantification (iBAQ) values were compared between all NuRD subunits and interactors. The iBAQ algorithm normalizes the total mass spec intensity for each protein according to the number of theoretically observable peptides [19]. This allows estimation of the relative abundance of large and small proteins detected in affinity purifications. Since the NuRD complex contains many paralogs that overlap at the peptide level, iBAQ values were summed for all paralogs (Table S1). Comparison of the iBAQ values relative to the GFP-MBD3 bait revealed that most core subunits of NuRD remain tightly bound to each other and to MBD3 despite the presence of high salt (1M NaCl) and NP-40 concentration (0.5%) in the wash steps (Fig 1E). Surprisingly, the core RBBP4 and -7 subunits were very sensitive to NP-40. Increasing the NP-40 concentration from 0.15% to 0.5% led to a decrease in the amount of RBBP4 and -7 associated with the complex (stoichiometry of 5.5 reduced to 4).\"" full coverage:Only protein-protein interactions curation depth:imex curation |
| NCBI Taxonomy identifier for the host organism | taxid:-1(in vitro) taxid:-1(In vitro) |
| Parameters of the interaction | - |
| Creation date | 2014/08/20 |
| Update date | 2014/10/16 |
| negative Boolean value | false |
| Feature(s) for interactor A | green fluorescent protein tag:?-? |
| Feature(s) for interactor B | - |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Identification by mass spectrometry |
| Participant identification method for interactor B | Identification by mass spectrometry |