| Unique identifier for interactor A | uniprotkb:Q86V81 |
| Unique identifier for interactor B | uniprotkb:Q96FV9 |
| Alternative identifier for interactor A | intact:EBI-347640 uniprotkb:O43672 |
| Alternative identifier for interactor B | intact:EBI-1765605 ensembl:ENSP00000261600.6 uniprotkb:B2RBP6 uniprotkb:Q64I72 uniprotkb:Q64I73 intact:EBI-28984554 uniprotkb:Q15219 |
| Aliases for A | psi-mi:thoc4_human(display_long) uniprotkb:ALYREF(gene name) psi-mi:ALYREF(display_short) uniprotkb:ALY(gene name synonym) uniprotkb:Ally of AML-1 and LEF-1(gene name synonym) uniprotkb:Aly/REF export factor(gene name synonym) uniprotkb:BEF(gene name synonym) uniprotkb:THOC4(gene name synonym) uniprotkb:Transcriptional coactivator Aly/REF(gene name synonym) uniprotkb:bZIP-enhancing factor BEF(gene name synonym) |
| Aliases for B | psi-mi:thoc1_human(display_long) uniprotkb:hTREX84(gene name synonym) uniprotkb:THOC1(gene name) psi-mi:THOC1(display_short) uniprotkb:HPR1(gene name synonym) uniprotkb:Nuclear matrix protein p84(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0007"(anti tag coimmunoprecipitation) |
| First author | Chang et al. (2013) |
| Identifier of the publication | imex:IM-22955 pubmed:23299939 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0914"(association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-9085174 imex:IM-22955-19 |
| Confidence score | intact-miscore:0.75 |
| Complex expansion | psi-mi:"MI:1060"(spoke expansion) |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Bait |
| Experimental role B | Prey |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f8a comment:"\"To address this, we IPed FLAG-Nxf1 and FLAG-Alyref from cells and gently eluted the IP from beads using FLAG peptide. A proportion of the IP was subjected to western analysis for TREX components (Figure 8A). In the FLAG-Alyref IP, we detected multiple TREX components including Uap56. FLAG-Nxf1 associated with multiple TREX components except Uap56, as expected since Uap56 is displaced from Alyref by Nxf1 (Hautbergue et al, 2008). We then took the eluate from the FLAG-Nxf1 IP and subjected it to a second round of IP with control (anti-HA), Alyref, Chtop and Thoc5 antibodies and analysed these IPs for TREX components. The control antibody used in the secondary IP failed to immunoprecipitate with any TREX component. In contrast, secondary IPs with Chtop, Alyref and Thoc5 antibodies revealed interaction with multiple TREX components but no Uap56 was detected as expected. These data are consistent with Nxf1, Chtop, Thoc5 and other TREX components existing as a single complex in vivo.\"" full coverage:Only protein-protein interactions curation depth:imex curation |
| NCBI Taxonomy identifier for the host organism | taxid:9606(human-293t) taxid:9606(Homo sapiens HEK293T embryonic kidney cell) |
| Parameters of the interaction | - |
| Creation date | 2014/01/08 |
| Update date | 2014/10/16 |
| negative Boolean value | false |
| Feature(s) for interactor A | flag tag:?-? |
| Feature(s) for interactor B | - |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Western blot |
| Participant identification method for interactor B | Western blot |