| Unique identifier for interactor A | uniprotkb:Q04724 |
| Unique identifier for interactor B | uniprotkb:P15884 |
| Alternative identifier for interactor A | intact:EBI-711424 uniprotkb:A8K495 ensembl:ENSP00000365682.3 uniprotkb:Q5T3G4 uniprotkb:Q969V9 |
| Alternative identifier for interactor B | intact:EBI-533224 uniprotkb:B4DT37 uniprotkb:G0LNT9 uniprotkb:G0LNU0 uniprotkb:G0LNU1 uniprotkb:G0LNU2 uniprotkb:G0LNU8 uniprotkb:G0LNU9 uniprotkb:G0LNV0 uniprotkb:G0LNV1 uniprotkb:B3KT62 uniprotkb:B3KUC0 uniprotkb:B4DUG3 uniprotkb:B7Z5M6 uniprotkb:G0LNU5 uniprotkb:H3BPQ1 uniprotkb:B7Z6Y1 uniprotkb:G0LNU4 uniprotkb:G0LNV2 ensembl:ENSP00000348374.4 ensembl:ENSP00000457649.1 uniprotkb:Q08AP3 uniprotkb:Q08AP2 uniprotkb:Q15440 uniprotkb:Q15441 uniprotkb:Q15439 |
| Aliases for A | psi-mi:tle1_human(display_long) uniprotkb:TLE1(gene name) psi-mi:TLE1(display_short) uniprotkb:Enhancer of split groucho-like protein 1(gene name synonym) uniprotkb:"E(Sp1) homolog"(gene name synonym) |
| Aliases for B | psi-mi:itf2_human(display_long) uniprotkb:Immunoglobulin transcription factor 2(gene name synonym) uniprotkb:SL3-3 enhancer factor 2(gene name synonym) uniprotkb:ITF2(gene name synonym) uniprotkb:SEF2(gene name synonym) uniprotkb:TCF4(gene name) psi-mi:TCF4(display_short) uniprotkb:BHLHB19(gene name synonym) uniprotkb:Class B basic helix-loop-helix protein 19(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0053"(fluorescence polarization spectroscopy) |
| First author | Chodaparambil et al. (2014) |
| Identifier of the publication | imex:IM-22946 pubmed:24596249 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0407"(direct interaction) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-9547025 imex:IM-22946-6 |
| Confidence score | intact-miscore:0.44 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Neutral component |
| Experimental role B | Neutral component |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f2c t1 f3b comment:"\"We used fluorescence anisotropy of labeled TLE120–156 to measure its affinity for purified mLEF1, hTCF1, xTCF3 and hTCF4 constructs lacking their HMG domains, a separate region that does not contribute to TLE interactions (Arce et al, 2009). TLE120-156 binds to TCF3 and TCF4 with KDs of 16 lm and 34 lM respectively, whereas it binds LEF1 and TCF1 with KDs of 195 lM and 1 mM (Fig 2C, Table 1).\"" comment:"\"The TLE120–156 L26D/I29D mutant selectively disrupts the tetramer without destroying the dimeric building block (Fig 1G), so we tested whether the dimer can still interact with TCF/LEF proteins. TLE120–156 mutant dimer binds to TCF3 and TCF4 with the same affinity as the wild-type tetramer (Fig 3B). This demonstrates that the dimer confers full binding to TCF3 and TCF4, and it suggests that the stoichiometry of TLE-TCF interaction is unlikely to be 4:1 TLE:TCF as reported (Daniels & Weis, 2005).\"" full coverage:Only protein-protein interactions curation depth:imex curation |
| NCBI Taxonomy identifier for the host organism | taxid:-1(in vitro) taxid:-1(In vitro) |
| Parameters of the interaction | kd:34.0x10^-6(molar) |
| Creation date | 2014/05/05 |
| Update date | 2014/10/16 |
| negative Boolean value | false |
| Feature(s) for interactor A | binding-associated region:20-156 |
| Feature(s) for interactor B | binding-associated region:1-300 |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Predetermined participant |
| Participant identification method for interactor B | Predetermined participant |