| Unique identifier for interactor A | uniprotkb:Q04724 |
| Unique identifier for interactor B | uniprotkb:P70062 |
| Alternative identifier for interactor A | intact:EBI-711424 ensembl:ENSP00000365682.3 uniprotkb:Q5T3G4 uniprotkb:Q969V9 uniprotkb:A8K495 |
| Alternative identifier for interactor B | intact:EBI-6259044 |
| Aliases for A | psi-mi:tle1_human(display_long) uniprotkb:TLE1(gene name) psi-mi:TLE1(display_short) uniprotkb:Enhancer of split groucho-like protein 1(gene name synonym) uniprotkb:"E(Sp1) homolog"(gene name synonym) |
| Aliases for B | psi-mi:t7l1a_xenla(display_long) uniprotkb:tcf7l1-a(gene name) psi-mi:tcf7l1-a(display_short) uniprotkb:tcf3(gene name synonym) uniprotkb:tcf3a(gene name synonym) uniprotkb:HMG box transcription factor 3-A(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0053"(fluorescence polarization spectroscopy) |
| First author | Chodaparambil et al. (2014) |
| Identifier of the publication | imex:IM-22946 pubmed:24596249 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:8355(xenla) taxid:8355("Xenopus laevis (African clawed frog)") |
| Interaction types | psi-mi:"MI:0407"(direct interaction) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-9547010 imex:IM-22946-5 |
| Confidence score | intact-miscore:0.44 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Neutral component |
| Experimental role B | Neutral component |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f2c t1 f3b comment:"\"We used fluorescence anisotropy of labeled TLE120–156 to measure its affinity for purified mLEF1, hTCF1, xTCF3 and hTCF4 constructs lacking their HMG domains, a separate region that does not contribute to TLE interactions (Arce et al, 2009). TLE120-156 binds to TCF3 and TCF4 with KDs of 16 lm and 34 lM respectively, whereas it binds LEF1 and TCF1 with KDs of 195 lM and 1 mM (Fig 2C, Table 1).\"" comment:"\"The TLE120–156 L26D/I29D mutant selectively disrupts the tetramer without destroying the dimeric building block (Fig 1G), so we tested whether the dimer can still interact with TCF/LEF proteins. TLE120–156 mutant dimer binds to TCF3 and TCF4 with the same affinity as the wild-type tetramer (Fig 3B). This demonstrates that the dimer confers full binding to TCF3 and TCF4, and it suggests that the stoichiometry of TLE-TCF interaction is unlikely to be 4:1 TLE:TCF as reported (Daniels & Weis, 2005).\"" curation depth:imex curation full coverage:Only protein-protein interactions |
| NCBI Taxonomy identifier for the host organism | taxid:-1(in vitro) taxid:-1(In vitro) |
| Parameters of the interaction | kd:16.0x10^-6(molar) |
| Creation date | 2014/05/05 |
| Update date | 2025/01/26 |
| negative Boolean value | false |
| Feature(s) for interactor A | binding-associated region:20-156 |
| Feature(s) for interactor B | binding-associated region:1-330 |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Predetermined participant |
| Participant identification method for interactor B | Predetermined participant |