Curation Details
Interaction ID: IM-22944-1

Unique identifier for interactor Auniprotkb:P29469
Unique identifier for interactor Buniprotkb:P24279
Alternative identifier for interactor Aintact:EBI-10533
uniprotkb:D6VPX7
Alternative identifier for interactor Bintact:EBI-10541
uniprotkb:D3DLL7
Aliases for Apsi-mi:mcm2_yeast(display_long)
uniprotkb:MCM2(gene name)
psi-mi:MCM2(display_short)
uniprotkb:YBL023C(locus name)
uniprotkb:YBL0438(orf name)
uniprotkb:Minichromosome maintenance protein 2(gene name synonym)
Aliases for Bpsi-mi:mcm3_yeast(display_long)
uniprotkb:MCM3(gene name)
psi-mi:MCM3(display_short)
uniprotkb:YEL032W(locus name)
uniprotkb:SYGP-ORF23(orf name)
uniprotkb:Minichromosome maintenance protein 3(gene name synonym)
Interaction detection methodspsi-mi:"MI:0040"(electron microscopy)
First authorOn et al. (2014)
Identifier of the publicationpubmed:24566989
imex:IM-22944
NCBI Taxonomy identifier for interactor Ataxid:559292(yeast)
taxid:559292(Saccharomyces cerevisiae)
NCBI Taxonomy identifier for interactor Btaxid:559292(yeast)
taxid:559292(Saccharomyces cerevisiae)
Interaction typespsi-mi:"MI:0915"(physical association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-9551191
imex:IM-22944-1
Confidence scoreintact-miscore:0.85
Complex expansionpsi-mi:"MI:1060"(spoke expansion)
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role AUnspecified role
Experimental role BUnspecified role
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:f1d f1e
comment:"\"DNA-bound Mcm2-7 complexes were released from the beads by EcoRI digestion as previously described (Remus et al, 2009) and applied directly onto carbon-coated copper grids for single particle EM analysis after negative staining. Fig 1D shows that Mcm2-7 double hexamers were readily observed in both the treated and untreated samples and, more significantly, Mcm2-7 single hexamers were not found in either sample. This indicates that DDK phosphorylation was not sufficient to cause efficient dissociation of the Mcm2-7 double hexamers despite the extensive phosphorylation of virtually all the Mcm4 and 6.\""
full coverage:Only protein-protein interactions
curation depth:imex curation
NCBI Taxonomy identifier for the host organismtaxid:-1(in vitro)
taxid:-1(In vitro)
Parameters of the interaction-
Creation date2014/06/17
Update date2014/10/16
negative Boolean valuefalse
Feature(s) for interactor A-
Feature(s) for interactor B-
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor APredetermined participant
Participant identification method for interactor BPredetermined participant