| Unique identifier for interactor A | uniprotkb:P10599 |
| Unique identifier for interactor B | uniprotkb:P18031 |
| Alternative identifier for interactor A | intact:EBI-594644 uniprotkb:B1ALW1 uniprotkb:O60744 uniprotkb:Q53X69 uniprotkb:Q96KI3 uniprotkb:Q9UDG5 intact:EBI-1042712 ensembl:ENSP00000363641.5 |
| Alternative identifier for interactor B | intact:EBI-968788 uniprotkb:Q5TGD8 uniprotkb:Q9BQV9 uniprotkb:Q9NQQ4 ensembl:ENSP00000360683.3 |
| Aliases for A | psi-mi:thio_human(display_long) uniprotkb:ATL-derived factor(gene name synonym) uniprotkb:Surface-associated sulphydryl protein(gene name synonym) uniprotkb:TXN(gene name) psi-mi:TXN(display_short) uniprotkb:TRDX(gene name synonym) uniprotkb:TRX(gene name synonym) uniprotkb:TRX1(gene name synonym) |
| Aliases for B | psi-mi:ptn1_human(display_long) uniprotkb:PTPN1(gene name) psi-mi:PTPN1(display_short) uniprotkb:PTP1B(gene name synonym) uniprotkb:Protein-tyrosine phosphatase 1B(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0007"(anti tag coimmunoprecipitation) |
| First author | Schwertassek et al. (2014) |
| Identifier of the publication | imex:IM-22939 pubmed:24976139 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0915"(physical association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-9635586 imex:IM-22939-2 |
| Confidence score | intact-miscore:0.40 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Prey |
| Experimental role B | Bait |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f5 comment:"\"As expected, exogenously applied H2O2 induced disulfide bond formation between PTP1B and the TRX1 trapping mutant (TRX1-C35S) (Figure 5A). To confirm that the catalytic cysteine of PTP1B is essential for disulfide bond formation with TRX1, we used a PTP1B mutant that lacks the catalytic cysteine, but adopts the conformation of the oxidized protein in a stable manner (PTP1B-CASA) [16]. Indeed, PTB1B-CASA failed to form a conjugate with TRX1-C35S (Figure 5B). Using this approach, we also detected TRX1(C35S)- PTP1B complex formation following stimulation of the cells with a physiological trigger, in this case insulin (Figure 5C). Again, conjugation depended on the catalytic cysteine of PTP1B (Figure 5D).\"" curation depth:imex curation full coverage:Only protein-protein interactions |
| NCBI Taxonomy identifier for the host organism | taxid:9606(human-293t) taxid:9606(Homo sapiens HEK293T embryonic kidney cell) |
| Parameters of the interaction | - |
| Creation date | 2014/06/30 |
| Update date | 2024/11/30 |
| negative Boolean value | false |
| Feature(s) for interactor A | trapping mutant:35-35 his tag:?-? |
| Feature(s) for interactor B | mutation disrupting interaction:215-216 flag tag:?-? |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Western blot |
| Participant identification method for interactor B | Western blot |