Curation Details
Interaction ID: IM-22938-3

Unique identifier for interactor Auniprotkb:Q13586
Unique identifier for interactor Buniprotkb:Q96D31
Alternative identifier for interactor Aintact:EBI-448878
ensembl:ENSP00000300737.4
uniprotkb:E9PQJ4
uniprotkb:Q8N382
Alternative identifier for interactor Bintact:EBI-2291476
uniprotkb:Q3MHV3
uniprotkb:Q6DHX2
uniprotkb:Q96BP7
uniprotkb:Q96K71
ensembl:ENSP00000482568.2
Aliases for Apsi-mi:stim1_human(display_long)
uniprotkb:STIM1(gene name)
psi-mi:STIM1(display_short)
uniprotkb:GOK(gene name synonym)
Aliases for Bpsi-mi:crcm1_human(display_long)
uniprotkb:ORAI1(gene name)
psi-mi:ORAI1(display_short)
uniprotkb:CRACM1(gene name synonym)
uniprotkb:TMEM142A(gene name synonym)
uniprotkb:Protein orai-1(gene name synonym)
uniprotkb:Transmembrane protein 142A(gene name synonym)
Interaction detection methodspsi-mi:"MI:0007"(anti tag coimmunoprecipitation)
First authorBalasuriya et al. (2014)
Identifier of the publicationpubmed:24996186
imex:IM-22938
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0915"(physical association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-9631553
imex:IM-22938-3
Confidence scoreintact-miscore:0.96
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role APrey
Experimental role BBait
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:f2b f3a
comment:"\"Co-transfected cells, either untreated or thapsigargin-treated, were solubilized, and the detergent extracts were subjected to anti-Myc immunoprecipitation followed by immunoblotting with anti-Myc (Orai1) and anti-HA (STIM1) antibodies. Orai1 was present in both samples, as expected (Fig. 2B), whereas STIM1 was coimmunopreciptated only from the extract of thapsigargin-treated cells, indicating that STIM1 associates with Orai1 only after store depletion.\""
full coverage:Only protein-protein interactions
curation depth:imex curation
NCBI Taxonomy identifier for the host organismtaxid:9606(human-tsa201)
taxid:9606(Homo sapiens tsa201 embryonic kidney cell)
Parameters of the interaction-
Creation date2014/06/23
Update date2014/10/16
negative Boolean valuefalse
Feature(s) for interactor Aha tag:?-?
Feature(s) for interactor Btandem tag:?-?
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor AAnti tag western blot
Participant identification method for interactor BAnti tag western blot